Influence of process parameters on yield, chemical composition and biological activities of Clinopodium nubigenum (Kunth) Kuntze (sunfo) extracts obtained by supercritical CO2 extraction and other techniques

IF 3.4 3区工程技术 Q2 CHEMISTRY, PHYSICAL

Journal of Supercritical Fluids Pub Date : 2024-12-12 DOI:10.1016/j.supflu.2024.106492

Lorena I. Jaramillo , Jalloul Bouajila , Edwin Vera , Séverine Camy

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引用次数: 0

Abstract

Sunfo volatile compounds (VC) were obtained by supercritical fluid extraction (SFE). The influence of temperature (40−60) °C, particle size (0.35–1) mm, flowrate (10−20) g/min and pressure (10−60) MPa on the yield was investigated. Composition, yield, antioxidant activity (AA) and total phenolic content (TPC) were compared with ultrasound assisted extraction (UAE), hydrodistillation (HD) and Soxhlet (SX). Extraction yields and major compounds obtained were: HD: 23.7 mg/g, thymol; SX: 244 mg/g, caryophyllene; 3′-hydroxyb-naphtoflavone; UAE: 227 mg/g, carvacrol, (z)-3-(3-ethoxy-4-hydroxy-phenyl)-2-phenylacrylic acid; SFE: 38.50 mg/g, thymol, carvacrol, (+-)-6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, 5-hydroxyflavone and icarrin. SX presented better AA (IC_50-DPPH =19.60 mg/mL) followed by UAE (IC_{50- DPPH} =48.13 mg/mL) and HD better TPC (184.07 mg GAE/g). Antifungal activity against Colletotrichum musae, Mucor racemosus and Fusarium verticillioides was assessed, minimal inhibitory concentration (MIC) was obtained. VC obtained had the most active antifungal activity against fungus (MIC: (400, 400, 825) mg/L).

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超临界CO2萃取等工艺参数对斜脚草（Kunth） Kuntze （sunfo）提取物收率、化学成分及生物活性的影响

采用超临界流体萃取法（SFE）得到三氟挥发物（VC）。考察了温度（40 ~ 60）℃、粒径（0.35 ~ 1）mm、流速（10 ~ 20）g/min、压力（10 ~ 60）MPa对产率的影响。比较了超声辅助提取法（UAE）、加氢蒸馏法（HD）和索氏法（SX）三种提取方法的成分、得率、抗氧化活性（AA）和总酚含量（TPC）。提取得率及主要化合物为：HD: 23.7 mg/g，百里香酚；SX: 244 mg/g，石竹烯；3 ' -hydroxyb-naphtoflavone;阿联酋：227 mg/g，香芹酚，(z)-3-（3-乙氧基-4-羟基苯基）-2-苯丙烯酸；SFE: 38.50 mg/g，百里香酚，香芹醇，(+-)-6-羟基-2,5,7,8-四甲基铬-2-羧酸，5 -羟黄酮和伊卡林。SX表现出较好的AA (IC50-DPPH =19.60 mg/mL)，其次是UAE （IC50-DPPH =48.13 mg/mL）和HD较好的TPC（184.07 mg GAE/g）。测定其对炭疽菌、总状毛霉和黄萎病镰刀菌的抑菌活性，获得最小抑菌浓度（MIC）。所得VC对真菌的抑菌活性最高（MIC分别为（400、400、825）mg/L）。

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来源期刊

Journal of Supercritical Fluids 工程技术-工程：化工

CiteScore

7.60

自引率

10.30%

发文量

236

审稿时长

56 days

期刊介绍： The Journal of Supercritical Fluids is an international journal devoted to the fundamental and applied aspects of supercritical fluids and processes. Its aim is to provide a focused platform for academic and industrial researchers to report their findings and to have ready access to the advances in this rapidly growing field. Its coverage is multidisciplinary and includes both basic and applied topics. Thermodynamics and phase equilibria, reaction kinetics and rate processes, thermal and transport properties, and all topics related to processing such as separations (extraction, fractionation, purification, chromatography) nucleation and impregnation are within the scope. Accounts of specific engineering applications such as those encountered in food, fuel, natural products, minerals, pharmaceuticals and polymer industries are included. Topics related to high pressure equipment design, analytical techniques, sensors, and process control methodologies are also within the scope of the journal.