Isolation, structure analysis and expression characterization of the Hexokinase gene family in Sorghum bicolor.

IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
3 Biotech Pub Date : 2025-01-01 Epub Date: 2024-12-20 DOI:10.1007/s13205-024-04190-5
Sen Li, Yansheng Liu, Xin'er Qin, Xiaofei He, Shaopeng Han, Yang Lv, Zhuying Deng, Gongjian Zeng, Xinqiang Gao, Yongfeng Hu, Xiangling Shen
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引用次数: 0

Abstract

Hexokinases (HXK) not only facilitate carbohydrate metabolism but also play important roles in sugar sensing in higher plants. HXK gene families have been extensively discussed in many plant species; however, comprehensive information regarding HXKs in sorghum remains unclear. To address this gap, we identified 7 putative sorghum HXKs (SbHXK1 to SbHXK7), and the features of their conserved domains, gene structure, evolutionary tree, and cis-acting elements were systematically characterized to reveal the evolutionary conservation between different plant species. Based on expression profiling, we found that different expression patterns of SbHXKs were associated with different physiological processes, including abiotic stresses. Further qRT-PCR verification under salt and sucrose treatment confirmed that SbHXK2, SbHXK4, and SbHXK5 may play very important roles under high osmotic pressure. Notably, SbHXKs are predominantly localized in the cytoplasm, in contrast to some rice and Arabidopsis HXKs, which are localized in chloroplasts or mitochondria, suggesting divergent roles for SbHXKs. In summary, our study provides a theoretical foundation for understanding the HXK gene family and offers fundamental insights of SbHXKs in sorghum.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04190-5.

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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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