A genus-specific R2R3 MYB transcription factor, CsMYB34, regulates galloylated catechin biosynthesis in Camellia sinensis.

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-12-15 DOI:10.1016/j.plaphy.2024.109401

Jianmei Xu, Jingyi Li, Yihao Liu, Peng Zheng, Shaoqun Liu, Binmei Sun

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Abstract

Galloylated catechins are the dominant polyphenols in Camellia sinensis (L.) O. Kuntze. The mechanisms responsible for accumulation of these specialized metabolites in tea plants remains unclear. This paper presents an extended member of subgroup 5 of transcription factors R2R3-MYB, CsMYB34, as a critical gene specifically regulating galloylated catechin biosynthesis. CsMYB34 has a TT2-type motif [VIRTKATRCSKVFIP]. Its transcription levels were positively correlated with galloylated catechin content in 19 tea varieties, with correlation coefficients ≥0.79. Suppression of CsMYB34 expression caused a significant decrease in galloylated catechin content, as well as reduced expression levels of the key galloylated catechin biosynthesis gene CsSCPL4. Yeast one-hybrid (Y1H), electrophoretic mobile shift assay (EMSA) and dual-luciferase reporter system (DLR) showed that CsMYB34 interacts directly with the promoter region of CsSCPL4, thereby upregulating its transcription. This research indicates that the CsMYB34 transcription factor selectively modulates the biosynthetic pathway of galloylated catechins, thereby offering a plausible rationale for the observed elevated levels of these compounds in tea leaves.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.