Comparison of highly sensitive, multiplex immunoassay platforms for streamlined clinical cytokine quantification.

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2025-01-01 Epub Date: 2024-12-20 DOI:10.1080/17576180.2024.2442190

Kevin McKinski, Huaping Tang, Kai Wang, Mary Birchler, Mike Wright

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引用次数: 0

Abstract

Introduction: Selecting the optimal platforms to quantitate cytokines is challenging due to varying performance and the plethora of options available.

Aims: To compare performance of three highly sensitive, multiplex assays on three different platforms - MSD S-plex, Olink Target 48, and Quanterix SP-X - to MSD V-plex which is widely used for quantitative cytokine assay.

Methods: Serum and stimulated plasma samples were analyzed across each platform. The proportion of quantifiable samples was compared for each analyte and correlation analyses were performed to relate the data. For MSD S-plex, parallelism and antibody pair knockdown experiments gauged specificity of the kit.

Results: MSD S-plex was the most sensitive multiplex platform followed by Olink Target 48, Quanterix SP-X, and MSD V-plex. Concentrations across platforms differed greatly for some cytokines, but all platforms showed strong correlation. Results for MSD S-plex were confirmed by parallelism and knockdown.

Conclusion: MSD S-plex should be a priority platform for ultra-sensitive assay. Olink Target 48 offers an enticing combination of sensitivity and multiplex capability that warrants consideration when many cytokines require quantitation. MSD V-plex, MSD S-plex and Olink quantitative assays offer high utility across drug development programs, but fit-for-purpose performance should be assessed on a per-analyte basis.

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流式临床细胞因子定量的高灵敏度、多重免疫分析平台的比较。

引言：由于细胞因子的性能各不相同，而且可供选择的平台也非常多，因此选择最佳平台来定量检测细胞因子是一项挑战：目的：比较三种不同平台（MSD S-plex、Olink Target 48 和 Quanterix SP-X）上三种高灵敏度的多重检测方法与广泛用于细胞因子定量检测的 MSD V-plex 的性能：方法：在每个平台上对血清和刺激血浆样本进行分析。方法：对每个平台的血清和刺激血浆样本进行分析，比较每种分析物的可定量样本比例，并进行相关性分析，将数据联系起来。对于 MSD S-plex，平行和抗体对敲除实验衡量了试剂盒的特异性：结果：MSD S-plex 是灵敏度最高的多重检测平台，其次是 Olink Target 48、Quanterix SP-X 和 MSD V-plex。不同平台上某些细胞因子的浓度差别很大，但所有平台都显示出很强的相关性。MSD S-plex 的结果得到了平行和基因敲除的证实：MSD S-plex 应成为超灵敏检测的优先平台。Olink Target 48 兼具灵敏度和多重检测能力，在需要定量检测多种细胞因子时值得考虑。MSD V-plex、MSD S-plex 和 Olink 定量检测在药物开发项目中具有很高的实用性，但应根据每个分析物评估其适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.