Upregulation of Peripheral Blood NLRP3 and IL-18 in Patients With Acute Kidney Injury in Sepsis and Its Clinical Significance

IF 3.1 4区医学 Q3 IMMUNOLOGY

Immunity, Inflammation and Disease Pub Date : 2024-12-18 DOI:10.1002/iid3.70113

Jing Zhou, Yibin Ye, Zhipeng Chen, Yong Liu, Baozheng Wu, Haiping Huang

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Abstract

Background

Sepsis-associated acute kidney injury (SA-AKI) is a common complication that can lead to renal failure in patients, significantly affecting the prognosis and survival of patients.

Objective

In this study, we aimed to evaluate the predictive value of NOD-like receptor protein 3 (NLRP3) and interleukin 18 (IL-18) in peripheral blood mononuclear cells (PBMCs) of SA patients for the occurrence of SA-AKI.

Material and Methods

We screened AKI-related data sets using the Gene Expression Omnibus (GEO) database and identified differentially expressed genes (DEGs) associated with AKI. KEGG and GO analysis were used to identify enriched molecular functions and pathways. The study included 62 SA patients admitted to the Department of Intensive Care Medicine of our hospital from February 2021 to June 2022, including 34 non-AKI cases and 28 AKI cases, and 25 healthy volunteers were used as the control group. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the levels of NLRP3 and IL-18 in PBMCs of the subjects.

Results

Bioinformatics analysis and experimental validation showed that the expression levels of NLRP3 and IL-18 were significantly upregulated in SA-AKI patients. In addition, the expressions of NLRP3 and IL-18 were positively correlated with APACHE II scores. ROC curve analysis revealed that NLRP3 and IL-18 have the potential to diagnose SA-AKI.

Conclusion

This study provides preliminary evidence for NLRP3 and IL-18 as potential diagnostic biomarkers for SA-AKI.

Abstract Image

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脓毒症急性肾损伤患者外周血NLRP3和IL-18表达上调及其临床意义

背景：脓毒症相关急性肾损伤（SA-AKI）是一种常见的并发症，可导致患者肾功能衰竭，严重影响患者的预后和生存。目的：本研究旨在评价SA患者外周血单个核细胞（PBMCs）中nod样受体蛋白3 （NLRP3）和白细胞介素18 （IL-18）对SA- aki发生的预测价值。材料和方法：我们使用Gene Expression Omnibus （GEO）数据库筛选AKI相关数据集，并鉴定与AKI相关的差异表达基因（DEGs）。KEGG和GO分析用于鉴定富集的分子功能和途径。本研究纳入我院重症医学科2021年2月至2022年6月收治的62例SA患者，其中非AKI患者34例，AKI患者28例，健康志愿者25例作为对照组。采用实时定量聚合酶链反应（RT-qPCR）检测受试者外周血NLRP3和IL-18水平。结果：生物信息学分析和实验验证表明，SA-AKI患者NLRP3和IL-18的表达水平显著上调。此外，NLRP3和IL-18的表达与APACHE II评分呈正相关。ROC曲线分析显示NLRP3和IL-18具有诊断SA-AKI的潜力。结论：本研究为NLRP3和IL-18作为SA-AKI的潜在诊断生物标志物提供了初步证据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunity, Inflammation and Disease Medicine-Immunology and Allergy

CiteScore

3.60

自引率

0.00%

发文量

146

审稿时长

8 weeks

期刊介绍： Immunity, Inflammation and Disease is a peer-reviewed, open access, interdisciplinary journal providing rapid publication of research across the broad field of immunology. Immunity, Inflammation and Disease gives rapid consideration to papers in all areas of clinical and basic research. The journal is indexed in Medline and the Science Citation Index Expanded (part of Web of Science), among others. It welcomes original work that enhances the understanding of immunology in areas including: • cellular and molecular immunology • clinical immunology • allergy • immunochemistry • immunogenetics • immune signalling • immune development • imaging • mathematical modelling • autoimmunity • transplantation immunology • cancer immunology