{"title":"Environmental release behavior, cell toxicity and intracellular distribution of novel biodegradable plastic materials","authors":"Liang Wen, Qian Hu, Yue Lv, Weitong Ding, Tian Yin, Hongjun Mao, Ting Wang","doi":"10.1016/j.envpol.2024.125554","DOIUrl":null,"url":null,"abstract":"In response to the increasingly severe issue of plastic waste, biodegradable plastics have garnered extensive attention as a potential alternative to traditional plastics. Among these materials, biodegradable plastics hold a dominant position. The objective of this study was to assess the environmental risks of five commercially available biodegradable plastics: polyglycolic acid (PGA), polylactic acid (PLA), poly(butylene succinate) (PBS), poly(butylene carbonate) (PBC), and poly(butylene adipate-co-terephthalate) (PBAT). The evaluation included their physical properties, microplastic release behavior, and cytotoxicity. In addition, the effect of age process on the environmental behavior of biodegradable plastic materials was further investigated. The results revealed that PGA and PBS exhibited lower risks in terms of microplastic release, whereas PLA demonstrated higher environmental mobility. Further cytotoxicity experiments indicated that PLA and PBS exerted significant toxic effects on human cell lines, including human normal liver cells (LO2), human monocytic leukemia cells (THP-1), human umbilical vein endothelial cells (HUVECs), and human colon carcinoma cells (Caco-2). Additionally, this study utilized Nile Red labeling to observe the co-culture system of PGA with THP-1 cells, uncovering that THP-1 cells gradually engulfed and internalized PGA microplastics over time. This finding provides new insights into the potential mechanism by which microplastics promote cell proliferation. Moreover, we also found that the aging process partially reduced the cytotoxicity of PGA, but had little effect on environmental mobility. Considering the comprehensive research findings, PGA is considered an ideal material for large-scale applications due to its low cytotoxicity and environmental risks. In contrast, the environmental safety of other types of plastics requires more comprehensive risk assessment to determine their suitability. This study provides significant scientific evidence for the environmental impact assessment of biodegradable plastics and plays a crucial role in promoting the development of sustainable plastic alternatives.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"38 1","pages":""},"PeriodicalIF":7.6000,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Pollution","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.envpol.2024.125554","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
In response to the increasingly severe issue of plastic waste, biodegradable plastics have garnered extensive attention as a potential alternative to traditional plastics. Among these materials, biodegradable plastics hold a dominant position. The objective of this study was to assess the environmental risks of five commercially available biodegradable plastics: polyglycolic acid (PGA), polylactic acid (PLA), poly(butylene succinate) (PBS), poly(butylene carbonate) (PBC), and poly(butylene adipate-co-terephthalate) (PBAT). The evaluation included their physical properties, microplastic release behavior, and cytotoxicity. In addition, the effect of age process on the environmental behavior of biodegradable plastic materials was further investigated. The results revealed that PGA and PBS exhibited lower risks in terms of microplastic release, whereas PLA demonstrated higher environmental mobility. Further cytotoxicity experiments indicated that PLA and PBS exerted significant toxic effects on human cell lines, including human normal liver cells (LO2), human monocytic leukemia cells (THP-1), human umbilical vein endothelial cells (HUVECs), and human colon carcinoma cells (Caco-2). Additionally, this study utilized Nile Red labeling to observe the co-culture system of PGA with THP-1 cells, uncovering that THP-1 cells gradually engulfed and internalized PGA microplastics over time. This finding provides new insights into the potential mechanism by which microplastics promote cell proliferation. Moreover, we also found that the aging process partially reduced the cytotoxicity of PGA, but had little effect on environmental mobility. Considering the comprehensive research findings, PGA is considered an ideal material for large-scale applications due to its low cytotoxicity and environmental risks. In contrast, the environmental safety of other types of plastics requires more comprehensive risk assessment to determine their suitability. This study provides significant scientific evidence for the environmental impact assessment of biodegradable plastics and plays a crucial role in promoting the development of sustainable plastic alternatives.
期刊介绍:
Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health.
Subject areas include, but are not limited to:
• Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies;
• Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change;
• Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects;
• Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects;
• Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest;
• New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.