Fluoride Dose-Response and Anticaries Effect of Herbal Antibacterial Agents and (Nano-)Hydroxyapatite on Dentin Caries: An In Vitro Study.

IF 2.9 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Caries Research Pub Date : 2024-12-16 DOI:10.1159/000542953

Richard Johannes Wierichs, Mowliharan Kuruparan, Abinaya Ruthiraswaran, Hendrik Meyer-Lueckel, Thiago Saads Carvalho, Samira Helena Niemeyer

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引用次数: 0

Abstract

Objectives: The aim of the study was to investigate the caries-preventive effect of fluoride-free toothpastes, containing either herbal agents or (nano-)hydroxyapatite.

Methods: Bovine dentin specimens each having a sound [ST], and a demineralized area [DT]) were prepared and randomly allocated to eleven groups (n=187). Treatments during pH-cycling (28 days;6x120min demineralization/day) were brushing 2x/day with: 0ppm F- [NaF0], 500ppm F- [NaF500], 1,100ppm F- [NaF1100], grape seed extract [GSE], (nano-)hydroxyapatite [nHA, HA], melaleuca oil [MO1, MO2, MO-CU] and propolis + myrrh [PM1, PM2] containing dentifrices. Dentifrice slurries were prepared with deionized water (1:3w/w). Differences in integrated mineral loss (∆∆Z) and lesion depth (∆LD) were evaluated before and after pH-cycling using transversal microradiography (TMR).

Results: The correlation between ΔΔZ/ΔLD and F- concentration (NaF0, NaF500, NaF1100) was strong for the DT (rΔΔZ,DT=0.681; p<0.001) and very strong for ST (rΔΔZ,ST=0.861; p<0.001); indicating a fluoride dose-response for both baseline substrate conditions. For ΔΔZDT and ΔLDDT only NaF1100 and GSE revealed significant differences compared with NaF0 (p<0.001, ANOVA). For ΔΔZST and ΔLDST significant differences could be found for NaF1100 and NaF500 compared to all fluoride-free groups (p≤0.002;ANOVA), without significant difference between fluoride-free groups (p=1.000;ANOVA). For DT and ST a hypermineralized surface layer and no surface loss could only be observed when fluoride was present.

Conclusion: A dose-response for fluoride concentrations was observed in this mild demineralization pH-cycling model. Fluoride-free dentifrices containing grape seed extract or melaleuca oil showed certain preventive effect against further progression of root caries lesions. However, surface loss was observed for all fluoride-free dentifrices.

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来源期刊

Caries Research 医学-牙科与口腔外科

CiteScore

6.30

自引率

7.10%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Caries Research'' publishes epidemiological, clinical and laboratory studies in dental caries, erosion and related dental diseases. Some studies build on the considerable advances already made in caries prevention, e.g. through fluoride application. Some aim to improve understanding of the increasingly important problem of dental erosion and the associated tooth wear process. Others monitor the changing pattern of caries in different populations, explore improved methods of diagnosis or evaluate methods of prevention or treatment. The broad coverage of current research has given the journal an international reputation as an indispensable source for both basic scientists and clinicians engaged in understanding, investigating and preventing dental disease.