Unusual frequency of Trypanosoma cruzi DTU TcI and predominance of hybrid lineages in Triatoma infestans before and after control interventions in the Argentinian Chaco.

Abstract

Transmission of Trypanosoma cruzi involves diverse hosts, vectors and parasitic genotypes, in different environments. In recent decades, the distribution of T. cruzi has altered due to urbanization of affected people and vectors. We implemented a longitudinal intervention program between 2015 and 2022 which aimed to suppress (peri)domestic Triatoma infestans in the municipality of Avia Terai (Chaco Province, Argentina), and found a marginal risk of domestic vector-borne transmission across the rural-to-urban gradient after interventions. Here, we identified the parasite Discrete Typing Units (DTUs) in infected T. infestans collected throughout the intervention program (37 insects pre-intervention and 7 post-intervention). Identification of DTUs was conducted by two methodologies, using DNA extracted from T. infestans rectal ampoules. We also assessed the association between blood-feeding sources and DTUs. Complete DTU identification was achieved in 48 % of samples. The hybrid lineages TcV or TcVI and their combinations predominated (72 %), followed by TcI (16 %) and mixed infections of TcI and hybrid lineages (14 %). Half (50 %) of the houses harbored TcI infected bugs either alone or mixed with TcII/TcV/TcVI. Humans predominated as the bloodmeal sources in all insects with identified DTU. All DTUs (TcI, TcV and TcII/TcV/TcVI) were recorded in both rural and peri-urban environments, with 62 % of the houses having more than one DTU. These results confirm the predominance of hybrid lineages in domestic transmission cycles of the Argentine Chaco. However, the finding of several triatomines infected with TcI both pre- and post-intervention raises the question of which host(s) are involved in its transmission.