Morphometric analysis of the female reproductive tract: influence of long-term inhalation of trace amounts of sevoflurane

IF 2.9 4区 生物学 Q3 CELL BIOLOGY
Haili Wang, Haibo Qu, Ailing Yang, Daru Guo, Lili Quan, Zhaodong Liu, Xiaoli Shi, Xibo Zhao, Yuanbo Feng, Tao Liu, Hua Pan
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引用次数: 0

Abstract

Sevoflurane is extensively employed as an inhalation anesthetic in medical practices, due to its promising pharmacokinetics. Conversely, the data regarding effects of prolonged exposure to trace amounts of sevoflurane on the female reproductive system is obscure. Therefore, this study aimed to investigate the reproductive toxicity and underlying mechanism of long-term sevoflurane inhalation in female rats. A total 60 SPF grade SD female rats were randomly alienated into four equal groups as control group (A), 50 ppm sevoflurane group (B), 150 ppm sevoflurane group (C), and 300 ppm sevoflurane groups (D). Ovaries and uterine organs were collected for gross as well as histopathological analysis, western blotting, and immuno-histochemistry evaluation. Results revealed that pregnancy rate, number of fetuses (fetal mice) and general body weight of group B, C, and D were substantially lower (P < 0.05), while were compared with control. On the contrary, estrous period in groups B, C, D was shortened noticeably (P < 0.05), and estrus interval and cycle were significantly longer (P < 0.05). In fact, the ovarian and uterine coefficients of group B, C and D were significantly reduced as compared with control. However, ovarian and uterine histomorphology remained normal in control group, while obvious pathological alterations were detected in groups B, C, and D. Although, the expression of SOD protein in the ovarian and uterine tissues of groups B, C, and D was significantly reduced (P < 0.05), in contrast to group A. However, the MDA protein expression increased significantly (P < 0.05) as compared with group A. While expression of apoptosis-related genes (Bcl2 and Bax) and humoral immunity related genes (IL-6, IL-10 and TNF-α) showed highest elevation in groups exposure with sevoflurane (p < 0.001) in comparison to control. Conclusively, long-term inhalation of trace amounts of sevoflurane is toxic to female reproductive system and can severely affect reproductive organs and fertility by induction of oxidative stress and apoptosis.

Graphical abstract

Abstract Image

女性生殖道形态分析:长期吸入微量七氟烷的影响
七氟醚由于其良好的药代动力学特性,在医疗实践中被广泛用作吸入麻醉剂。相反,关于长期接触微量七氟醚对女性生殖系统影响的数据是模糊的。因此,本研究旨在探讨长期吸入七氟醚对雌性大鼠的生殖毒性及其机制。选取SPF级SD雌性大鼠60只,随机分为对照组(A)、50 ppm七氟醚组(B)、150 ppm七氟醚组(C)和300 ppm七氟醚组(D) 4组。采集卵巢和子宫器官进行大体、组织病理学分析、免疫印迹和免疫组织化学评价。结果显示,与对照组相比,B、C、D组的妊娠率、胎数(胎鼠)和总体重均显著降低(P < 0.05)。相反,B、C、D组发情期明显缩短(P < 0.05),发情间隔和发情周期明显延长(P < 0.05)。事实上,B组、C组和D组的卵巢和子宫系数与对照组相比均显著降低。B、C、D组卵巢和子宫组织形态学基本正常,B、C、D组卵巢和子宫组织中SOD蛋白表达较a组明显降低(P < 0.05)。与a组相比,MDA蛋白表达显著升高(P < 0.05),七氟醚暴露组细胞凋亡相关基因(Bcl2、Bax)和体液免疫相关基因(IL-6、IL-10、TNF-α)表达升高最高(P < 0.001)。综上所述,长期吸入微量七氟醚对女性生殖系统具有毒性,可通过诱导氧化应激和细胞凋亡严重影响生殖器官和生育能力。图形抽象
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来源期刊
Journal of Molecular Histology
Journal of Molecular Histology 生物-细胞生物学
CiteScore
5.90
自引率
0.00%
发文量
68
审稿时长
1 months
期刊介绍: The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.
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