Knocking Down LncRNA-GAS5 Restores the Inhibition of Cuprotosis Protein FDX-1 by Acinetobacter baumannii.

Abstract

This study investigated the regulatory effect of LncRNA-GAS5 on FDX-1 in HEK293T cells by Acinetobacter baumannii. Transfected LncRNA-GAS5 overexpressing or knocking down plasmids into HEK293T cells, and the expression of FDX-1 was detected by Western blotting. A. baumannii inhibited the expression of FDX-1. Overexpression of LncRNA-GAS5 inhibited the expression of FDX-1, while knocking down LncRNA-GAS5 increased the expression of FDX-1. Overexpression of LncRNA-GAS5 further enhanced the inhibitory effect of A. baumannii on FDX-1, while knocking down LncRNA-GAS5 restored the inhibitory effect of A. baumannii on FDX-1. LncRNA-GAS5 regulates the inhibitory effect of A. baumannii on FDX-1 in HEK293T cells.