Application of Optical Coherence Tomography (OCT) to Evaluate the Adverse Effects of 2,4-D Dimethylammonium on Morphological Changes in Riceland Prawn (Macrobrachium lanchesteri).

Environmental toxicology and pharmacology Pub Date : 2024-12-10 DOI:10.1016/j.etap.2024.104608

Chutima Thanomsit, Sugunya Kumla, Jadsada Saetiew, Jiraporn Saenjae, Phochit Nanthanawat, Jakkaphun Nanuam, Panomsak Meemon

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Abstract

2,4-D dimethylammonium, a widely used herbicide, accumulates in aquatic animals, posing risks to both their populations and human consumers. This study evaluated the effects of 2,4-D on Riceland prawn (Macrobrachium lanchesteri) using Optical Coherence Tomography (OCT) to assess morphological changes. The activity of acetylcholinesterase (AChE), catalase (CAT), and glutathione S-transferase (GST) was also measured. Toxicity increased with both concentration and exposure duration. OCT revealed swelling in the gill region, tissue abnormalities, and muscle damage in the abdominal somite, telson, and uropod, along with tissue separation and degradation. Histological analysis confirmed muscle degeneration, hemocyte infiltration, and swelling. AChE expression was undetectable at 400 and 450µL/L after 96hours, with a molecular weight of 71kDa. CAT and GST activities decreased at higher herbicide concentrations, further demonstrating the harmful effects of 2,4-D on prawn health.

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2,4-D 二甲基铵是一种广泛使用的除草剂，会在水生动物体内蓄积，对其种群和人类消费者都构成风险。本研究利用光学相干断层扫描（OCT）技术评估了 2,4-D 对里氏对虾（Macrobrachium lanchesteri）的形态变化。此外，还测量了乙酰胆碱酯酶（AChE）、过氧化氢酶（CAT）和谷胱甘肽 S 转移酶（GST）的活性。毒性随浓度和接触时间的延长而增加。OCT 显示鳃区肿胀、组织异常，腹部体节、鱼尾和尿脚肌肉受损，组织分离和退化。组织学分析证实了肌肉变性、血细胞浸润和肿胀。在 400 和 450µL/L 浓度下，96 小时后检测不到 AChE 的表达，其分子量为 71kDa。除草剂浓度越高，CAT 和 GST 活性越低，这进一步证明了 2,4-D 对对虾健康的有害影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Environmental toxicology and pharmacology

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