Herbal Multiomics Provide Insights into Gene Discovery and Bioproduction of Triterpenoids by Engineered Microbes

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-12 DOI:10.1021/acs.jafc.4c08372

Feng Zhang, Xuemi Hao, Jia Liu, Hongping Hou, Shilin Chen, Caixia Wang

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引用次数: 0

Abstract

Triterpenoids are natural products found in plants that exhibit industrial and agricultural importance. Triterpenoids are typically synthesized through two main pathways: the mevalonate (MVA) and methylerythritol 4-phosphate (MEP) pathways. They then undergo structural diversification with the help of squalene cyclases (OSCs), cytochrome P450 monooxygenases (P450s), UDP glycosyltransferases (UGTs), and acyltransferases (ATs). Advances in multiomics technologies for herbal plants have led to the identification of novel triterpenoid biosynthetic pathways. The application of various analytical techniques facilitates the qualitative and quantitative analysis of triterpenoids. Progress in synthetic biology and metabolic engineering has also facilitated the heterologous production of triterpenoids in microorganisms, such as Escherichia coli and Saccharomyces cerevisiae. This review summarizes recent advances in biotechnological approaches aimed at elucidating the complex pathway of triterpenoid biosynthesis. It also discusses the metabolic engineering strategies employed to increase the level of triterpenoid production in chassis cells.

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三萜类化合物是植物中的天然产物，在工业和农业方面具有重要意义。三萜类化合物通常通过两种主要途径合成：甲羟戊酸（MVA）途径和季戊四醇磷酸酯（MEP）途径。然后，它们在角鲨烯环化酶（OSCs）、细胞色素 P450 单氧化酶（P450s）、UDP 糖基转移酶（UGTs）和酰基转移酶（ATs）的帮助下进行结构多样化。草本植物多组学技术的进步已导致新型三萜类化合物生物合成途径的鉴定。各种分析技术的应用有助于对三萜类化合物进行定性和定量分析。合成生物学和代谢工程方面的进展也促进了三萜类化合物在大肠杆菌和酿酒酵母等微生物中的异源生产。本综述总结了旨在阐明三萜类化合物生物合成复杂途径的生物技术方法的最新进展。它还讨论了为提高底盘细胞中三萜类化合物的生产水平而采用的代谢工程策略。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.