In vitro susceptibility testing of essential oils and their main components against antifungal-resistant Malassezia pachydermatis.

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Journal of Veterinary Medical Science Pub Date : 2024-12-11 DOI:10.1292/jvms.24-0342

Honoka Nojo, Nobuo Murayama, Sanae A Ishijima, Rui Kano

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Abstract

Canine Malassezia dermatitis (CMD) and otitis externa are generally treated by antifungal drugs. However, azole-resistant strains have been isolated from canine skin and ear canals worldwide. Phytochemicals isolated from essential oils are effective alternatives for inhibiting Malassezia pachydermatis. To evaluate the usefulness of phytochemicals against azole-resistant isolates, we performed in vitro susceptibility testing using the phytochemicals carvacrol, citral, and thymol. Eight antifungal-resistant isolates were obtained from 7 cases of dermatitis and 1 case of otitis externa during 2022 and 2023 from dogs in Tokyo and Kanagawa, Japan. Fungal susceptibility to carvacrol, citral, and thymol was assessed using the modified broth microdilution method. The minimum inhibitory concentrations (MICs) of the phytochemicals in all isolates were as follows: 0.03 to 0.125% for carvacrol; 0.03 to 0.125% for thymol; and 0.03% to 0.125% for citral. Based on these results, carvacrol, citral, and thymol appear to be effective against azole-resistant strains. The phytochemicals appear to be effective for treating antifungal-resistant cases of CMD and otitis externa.

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犬马拉色菌皮炎（CMD）和外耳道炎一般采用抗真菌药物治疗。然而，世界各地的犬皮肤和耳道中都分离出了耐唑菌株。从精油中分离出的植物化学物质是抑制马拉色菌的有效替代品。为了评估植物化学物质对耐唑分离物的作用，我们使用植物化学物质香芹酚、柠檬醛和百里酚进行了体外药敏试验。在 2022 年和 2023 年期间，我们从日本东京和神奈川的 7 例皮炎和 1 例外耳道炎病例中获得了 8 个抗真菌分离株。采用改良肉汤微稀释法评估了真菌对香芹酚、柠檬醛和百里酚的敏感性。植物化学物质对所有分离物的最小抑菌浓度（MICs）如下：香芹酚的最低抑菌浓度为 0.03% 至 0.125%；百里酚的最低抑菌浓度为 0.03% 至 0.125%；柠檬醛的最低抑菌浓度为 0.03% 至 0.125%。根据这些结果，香芹酚、柠檬醛和百里酚似乎对抗唑菌株有效。这些植物化学物质似乎对治疗对抗真菌有耐药性的慢性中耳炎和外耳道炎有效。

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来源期刊

Journal of Veterinary Medical Science 农林科学-兽医学

CiteScore

2.30

自引率

8.30%

发文量

230

审稿时长

9-18 weeks

期刊介绍： JVMS is a peer-reviewed journal and publishes a variety of papers on veterinary science from basic research to applied science and clinical research. JVMS is published monthly and consists of twelve issues per year. Papers are from the areas of anatomy, physiology, pharmacology, toxicology, pathology, immunology, microbiology, virology, parasitology, internal medicine, surgery, clinical pathology, theriogenology, avian disease, public health, ethology, and laboratory animal science. Although JVMS has played a role in publishing the scientific achievements of Japanese researchers and clinicians for many years, it now also accepts papers submitted from all over the world.