Polymorphisms in the type A blaZ gene as determinants of the cefazolin inoculum effect in Staphylococcus aureus.

IF 4.1 2区 医学 Q2 MICROBIOLOGY
Shinwon Lee, Sohee Park, Jeong Nam Kim, Soon Ok Lee, Jeong Eun Lee, Sun Hee Lee
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引用次数: 0

Abstract

Plasmids containing the wild-type (WT) or mutant type A blaZ (blaZA) gene (mutations at codons 226, 229, or both 226 and 229) were constructed and transformed into Staphylococcus aureus RN4220, yielding the strains WT-blaZ, M226-blaZ, M229-blaZ, and MB-blaZ. The high-inoculum cefazolin MIC was significantly lower in MB-blaZ and M226-blaZ than in WT-blaZ but not in M229-blaZ, suggesting that the single nucleotide polymorphism at codon 226 in blaZA contributes to the cefazolin inoculum effect.

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来源期刊
CiteScore
10.00
自引率
8.20%
发文量
762
审稿时长
3 months
期刊介绍: Antimicrobial Agents and Chemotherapy (AAC) features interdisciplinary studies that build our understanding of the underlying mechanisms and therapeutic applications of antimicrobial and antiparasitic agents and chemotherapy.
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