Nadezhda V Shilova, Svetlana M Polyakova, Alexey Yu Nokel, Alexander D Lipatnikov, Elena A Gordeeva, Marina V Lavrenteva, Nicolai V Bovin
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引用次数: 0
Abstract
Fluorescently labeled and conjugated (strept)avidins are widely used for imaging biotinylated molecules in immunological assays and histochemistry. We showed that besides biotin, these proteins bind glycans, including fragments of mammalian glycoproteins and glycolipids, in particular, ABO blood group antigens, oligolactosamines, and 6-O-sulfated oligosaccharides. This interaction is inhibited in a dose-dependent manner by micromolar concentrations of polymeric, but not monomeric, glycan conjugates (i.e., requires polyvalence). Taking into account the cluster organization of cell glycans (glycoproteins and glycolipids), the ability of (strept)avidins to bind glycans might be a source of errors in the analysis of carbohydrate-containing samples, which can be prevented by avoiding a large excess of (strept)avidin in analytical systems.
荧光标记和共轭（链状）亲和素在免疫分析和组织化学中广泛用于成像生物素化分子。我们发现除了生物素外，这些蛋白还结合聚糖，包括哺乳动物糖蛋白和糖脂的片段，特别是ABO血型抗原、低聚乳糖胺和6- o -硫酸化低聚糖。这种相互作用以剂量依赖的方式被微摩尔浓度的聚合物（而不是单体）聚糖偶联物（即需要多价）抑制。考虑到细胞聚糖（糖蛋白和糖脂）的簇状组织，（strept）亲和素结合聚糖的能力可能是分析含碳水化合物样品时误差的来源，这可以通过避免分析系统中大量过量的（strept）亲和素来防止。
期刊介绍：
Biochemistry (Moscow) is the journal that includes research papers in all fields of biochemistry as well as biochemical aspects of molecular biology, bioorganic chemistry, microbiology, immunology, physiology, and biomedical sciences. Coverage also extends to new experimental methods in biochemistry, theoretical contributions of biochemical importance, reviews of contemporary biochemical topics, and mini-reviews (News in Biochemistry).
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