Synergistic effects of platelet-rich fibrin and photobiomodulation on bone regeneration in MC3T3-E1 Preosteoblasts

IF 3.1 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy Pub Date : 2025-02-01 DOI:10.1016/j.pdpdt.2024.104436

Ling-Hung Chou , Tzu-Sen Yang , Pei-Chun Wong , Ying-Chun Chen

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引用次数: 0

Abstract

Background

Platelet-rich fibrin (PRF) and Photobiomodulation (PBM) are established methods for promoting bone healing. PRF enhances cell proliferation and migration due to its rich concentration of growth factors, while PBM stimulates tissue repair through mitochondrial activation. Despite their efficacies, no in-depth studies have explored the synergistic effects of combining PRF and PBM.

Methods

PRF was prepared at 50 % and 100 % concentrations, and PBM was applied using an 830 nm near-infrared laser at a dose of 5 J/cm². Cell viability, migration, and calcium deposition were assessed over seven and fourteen days.

Results

The combination of PRF and PBM significantly improved cell viability, migration, and calcium deposition, with the most notable effects observed after seven and fourteen days. However, a slight decrease in calcium deposition was noted in the 100 % PRF combined with the PBM group, suggesting a potential feedback mechanism at higher PRF concentrations.

Conclusions

This study explores the synergistic effects of PRF and PBM, offering new insights into optimizing bone tissue engineering strategies. The findings highlight the potential of this combined approach in enhancing bone regeneration, although further research is needed to refine the optimal conditions for these therapies.

查看原文本刊更多论文

富血小板纤维蛋白和光生物调节对MC3T3-E1成骨前细胞骨再生的协同作用。

背景：富血小板纤维蛋白（PRF）和光生物调节（PBM）是促进骨愈合的常用方法。PRF通过其丰富的生长因子浓度促进细胞增殖和迁移，PBM通过线粒体激活刺激组织修复。PRF与PBM的协同效应虽有疗效，但尚未有深入的研究探讨其协同效应。方法：制备50%和100%浓度的PRF，用830 nm近红外激光照射PBM，剂量为5 J/cm²。在7天和14天内评估细胞活力、迁移和钙沉积。结果：PRF和PBM联合用药可显著提高细胞活力、迁移能力和钙沉积能力，以第7天和第14天效果最显著。然而，在100% PRF与PBM组合组中，钙沉积略有减少，这表明在更高的PRF浓度下存在潜在的反馈机制。结论：本研究首次探讨了PRF和PBM的协同效应，为优化骨组织工程策略提供了新的见解。研究结果强调了这种联合方法在促进骨再生方面的潜力，尽管需要进一步的研究来完善这些治疗的最佳条件。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Photodiagnosis and Photodynamic Therapy ONCOLOGY-

CiteScore

5.80

自引率

24.20%

发文量

509

审稿时长

50 days

期刊介绍： Photodiagnosis and Photodynamic Therapy is an international journal for the dissemination of scientific knowledge and clinical developments of Photodiagnosis and Photodynamic Therapy in all medical specialties. The journal publishes original articles, review articles, case presentations, "how-to-do-it" articles, Letters to the Editor, short communications and relevant images with short descriptions. All submitted material is subject to a strict peer-review process.