Plant growth-promoting Bacillus amyloliquefaciens orchestrate homeostasis under nutrient deficiency exacerbated drought and salinity stress in Oryza sativa L. seedlings.

Abstract

Main conclusion: Nutrient deficiency intensifies drought and salinity stress on rice growth. Bacillus amyloliquefaciens inoculation provides resilience through modulation in metabolic and gene regulation to enhance growth, nutrient uptake, and stress tolerance. Soil nutrient deficiencies amplify the detrimental effects of abiotic stresses, such as drought and salinity, creating substantial challenges for overall plant health and crop productivity. Traditional methods for developing stress-resistant varieties are often slow and labor-intensive. Previously, we demonstrated that plant growth-promoting rhizobacteria Bacillus amyloliquefaciens strain SN13 effectively alleviates stress induced by sub-optimum nutrient conditions in rice. In this study, we evaluated the effectiveness of SN13 in reducing the compounded impacts of drought and salinity under varying nutrient regimes in rice seedlings. The results demonstrated that PGPR inoculation not only improved the growth parameters, nutrient content, and physio-biochemical characteristics under nutrient-limited conditions, but also reduced the oxidative stress markers. The altered expression of stress-related and transcription factor genes (USP, DEF, CYP450, GST, MYB, and bZIP) revealed the regulatory effect of PGPR in enhancing stress tolerance through these genes. GC-MS-based untargeted metabolomic analysis revealed that PGPR significantly influenced various metabolic pathways, including galactose metabolism, fructose and mannose metabolism, and fatty acid biosynthesis pathways, suggesting that PGPR affects both energy production and stress-protective mechanisms, facilitating better growth and survival of rice seedlings.