An efficient method for visualizing the plaques of Bdellovibrio bacteriovorus

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Qian Zhao, Jiangong Xu, Meirong Song, Kui Zhu
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引用次数: 0

Abstract

Bdellovibrio bacteriovorus, an obligate predator of Gram-negative bacteria, has emerged as a promising natural antibiotic to combat the escalating threat of antibiotic resistance. Plaque forming units (PFU) counting is commonly used to determine the viable numbers of B. bacteriovorus. However, nearly three-days incubation is always necessary for getting the single, obvious plaques on the double-layer agar plate. This time-consuming procedure greatly impedes the purification and enumeration efficiency of B. bacteriovorus. Until now, no simplified method has been developed to address this issue. In this work, we evaluated the advantages of using fluorescent prey to enhance the visualization of the B. bacteriovorus plaques. Our work reveals that the regular single plaques are easily observed under the enhanced background of fluorescent prey lawn in one and a half days, reducing nearly half of the time consumption in the purification and enumeration of B. bacteriovorus.

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来源期刊
Archives of Microbiology
Archives of Microbiology 生物-微生物学
CiteScore
4.90
自引率
3.60%
发文量
601
审稿时长
3 months
期刊介绍: Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.
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