{"title":"The impact of combined thymol and rosmarinic acid on the intestinal microbiota and barrier function of the piglets challenged by Escherichia. coli K88","authors":"Runlin Li, Xuedong Ding, Mingkang Lei, Panpan Li, Ilias Giannenas, Jing Wang, Weiyun Zhu","doi":"10.1016/j.aninu.2024.11.008","DOIUrl":null,"url":null,"abstract":"It has been found that thymol (Thy) and rosmarinic acid (Ros-A) improve the growth performance of piglets and relieve intestinal inflammation in animals. The effects of Thy and Ros-A separately or in combination (Thy×Ros-A) on the intestinal function and health of piglets challenged with <ce:italic>Escherichia coli</ce:italic> K88 (<ce:italic>E. coli</ce:italic> K88) were investigated. A total of 30 piglets aged 21 d were assigned to 5 groups (<ce:italic>n =</ce:italic> 6). The control (Con) and K88 groups piglets received a basal diet, while the Thy, Ros-A, and Thy×Ros-A groups were fed a basal diet supplemented with 500 mg/kg Thy, 500 mg/kg Ros-A, and 250 mg/kg Thy + 250 mg/kg Ros-A, respectively. On the 19th and 20th day, piglets in the K88, Thy, Ros-A, and Thy×Ros-A groups were orally administered 10 mL of phosphate-buffered saline (PBS) containing approximately 1 × 10<ce:sup loc=\"post\">9</ce:sup> colony-forming units/mL of <ce:italic>E. coli</ce:italic> K88, while the Con group received an equal volume of PBS. The results showed that the Thy×Ros-A treatment reduced the damage to ileal villi induced by the <ce:italic>E. coli</ce:italic> K88 challenge, leading to longer villi in the ileum (<ce:italic>P</ce:italic> < 0.05). Thy and Ros-A modulated the composition of the ileal microbiota. Compared to the K88 group, the Thy×Ros-A group had a higher abundance of <ce:italic>Lactobacillus</ce:italic> and <ce:italic>Romboutsia</ce:italic>, while <ce:italic>Escherichia-Shigella</ce:italic> and <ce:italic>Desulforvibrio</ce:italic> were lower (<ce:italic>P</ce:italic> < 0.05). Additionally, the Thy×Ros-A group showed elevated levels of gene and protein expressions for zonula occludens-1, occludin, and claudin-1 compared to the K88 group (<ce:italic>P</ce:italic> < 0.05). In conclusion, combining Thy and Ros-A reduced ileal damage and relieved the inflammation in weaned piglets challenged with <ce:italic>E. coli</ce:italic> K88 by regulating intestinal microflora and improving barrier function.","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"139 1","pages":""},"PeriodicalIF":6.1000,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Nutrition","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.aninu.2024.11.008","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
Abstract
It has been found that thymol (Thy) and rosmarinic acid (Ros-A) improve the growth performance of piglets and relieve intestinal inflammation in animals. The effects of Thy and Ros-A separately or in combination (Thy×Ros-A) on the intestinal function and health of piglets challenged with Escherichia coli K88 (E. coli K88) were investigated. A total of 30 piglets aged 21 d were assigned to 5 groups (n = 6). The control (Con) and K88 groups piglets received a basal diet, while the Thy, Ros-A, and Thy×Ros-A groups were fed a basal diet supplemented with 500 mg/kg Thy, 500 mg/kg Ros-A, and 250 mg/kg Thy + 250 mg/kg Ros-A, respectively. On the 19th and 20th day, piglets in the K88, Thy, Ros-A, and Thy×Ros-A groups were orally administered 10 mL of phosphate-buffered saline (PBS) containing approximately 1 × 109 colony-forming units/mL of E. coli K88, while the Con group received an equal volume of PBS. The results showed that the Thy×Ros-A treatment reduced the damage to ileal villi induced by the E. coli K88 challenge, leading to longer villi in the ileum (P < 0.05). Thy and Ros-A modulated the composition of the ileal microbiota. Compared to the K88 group, the Thy×Ros-A group had a higher abundance of Lactobacillus and Romboutsia, while Escherichia-Shigella and Desulforvibrio were lower (P < 0.05). Additionally, the Thy×Ros-A group showed elevated levels of gene and protein expressions for zonula occludens-1, occludin, and claudin-1 compared to the K88 group (P < 0.05). In conclusion, combining Thy and Ros-A reduced ileal damage and relieved the inflammation in weaned piglets challenged with E. coli K88 by regulating intestinal microflora and improving barrier function.
Animal NutritionAgricultural and Biological Sciences-Animal Science and Zoology
CiteScore
7.40
自引率
3.20%
发文量
172
审稿时长
12 weeks
期刊介绍:
Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to nutrition, and more applied aspects of animal nutrition, such as raw material evaluation, feed additives, nutritive value of novel ingredients and feed safety.