Molecular and ultrastructural characteristics of virulent and attenuated vaccine strains of goose parvovirus LIV-22

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2024-12-08 DOI:10.1007/s00705-024-06188-z

Ezekiel S. Oloruntimehin, Sofya I. Volodina, Ivan V. Patraman, Grigory A. Nadtochey, Alexander S. Malogolovkin

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Abstract

The disease caused by goose parvovirus (GPV) affects young goslings and ducks and leads to substantial losses for farmers due to high mortality rates, reaching 70-100% in naive flocks. Here, we present the results of a study focusing on the historical virulent GPV LIV-22 strain, which was isolated in the USSR in 1972. An attenuated GPV LIV-22 vaccine strain that was generated by continuous passaging in goose embryonic fibroblasts was also studied. Phylogenetic analysis placed both GPV LIV-22 strains in the classical GPV group, close to the vaccine and low-pathogenic strains. However, several individual changes in the GPV LIV-22 VP1 gene highlight the uniqueness of the evolution and adaptation mechanism of GPV LIV-22 strains. Transmission electron microscopy (TEM) revealed severe ultrastructural changes in goose hepatocytes and enterocytes as early as 24-48 h postinfection, confirming abrupt GPV pathogenesis. This description of some of the essential characteristics of the GPV LIV-22 virulent and vaccine strain will be useful for studying GPV evolution and molecular pathogenesis.

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鹅细小病毒LIV-22强毒株和弱毒株的分子和超微结构特征

鹅细小病毒（GPV）引起的疾病影响雏鹅和鸭，由于高死亡率给农民造成重大损失，在未成熟的鸡群中达到70-100%。在这里，我们介绍了对1972年在苏联分离的历史毒性GPV LIV-22株的研究结果。对鹅胚成纤维细胞连续传代产生的GPV减毒株LIV-22进行了研究。系统发育分析表明，这两种GPV LIV-22菌株均属于经典GPV组，接近疫苗和低致病性菌株。然而，GPV LIV-22 VP1基因的几个个体变化凸显了GPV LIV-22菌株进化和适应机制的独特性。透射电镜（TEM）显示，早在感染后24-48 h，鹅肝细胞和肠细胞就发生了严重的超微结构变化，证实了GPV的突变发病。对GPV LIV-22毒力株和疫苗株的一些基本特征的描述将有助于研究GPV的进化和分子发病机制。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.