A qPCR Assay for the Quantification of the Overwintering Chasmothecia of Erysiphe necator in Grapevine Bark.

IF 2.6 2区农林科学 Q2 PLANT SCIENCES

Phytopathology Pub Date : 2024-12-05 DOI:10.1094/PHYTO-04-24-0126-R

Othmane Taibi, Margherita Furiosi, Maela León, Elisa González-Domínguez, Vittorio Rossi, Mónica Berbegal

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引用次数: 0

Abstract

Powdery mildew (PM) disease causes serious losses in Mediterranean vineyards, where suitable environmental conditions promote conidial infections. The frequency and intensity of these infections are directly linked to the amount of primary Erysiphe necator inoculum, i.e., the chasmothecia embedded in the trunk. In this study, we set up a protocol to extract and quantify E. necator chasmothecia in grapevine bark samples based on a quantitative polymerase chain reaction (qPCR) assay. Moreover, we observed PM severity and ascocarp production on leaves in the first season and primary infection in the following season in different grapevine cvs. with known PM susceptibility levels. The qPCR analysis showed a significant relationship between E. necator DNA concentration in bark samples and primary infection (R² = 0.970) and disease severity development (R² = 0.776), as well as chasmothecia development on leaves (R² = 0.455). The results demonstrate that this methodology can be used for quantifying chasmothecia, improving current methods based on visual counting, proving the interrelationships between PM epidemics and chasmothecia, as well as refining PM disease prediction models and subsequent fungicide application. Rapid and easy quantification of ascosporic inoculum will greatly facilitate the reconciliation of control actions to the risks posed by greatly differing levels of ascosporic inoculum.

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用qPCR方法定量测定葡萄树皮中丹参越冬菌的数量。

白粉病（PM）在地中海葡萄园造成严重损失，适宜的环境条件促进分孢子感染。这些感染的频率和强度直接与初级接种量有关，即埋在树干中的裂口菌。在本研究中，我们建立了一种基于定量聚合酶链反应（qPCR）方法提取和定量葡萄树皮样品中necator chasmothecia的方法。此外，我们还观察了不同葡萄品种在第1季和第2季的PM严重程度和叶片上的ascocarp产量。已知PM易感性水平qPCR分析显示，树皮样品中necator DNA浓度与原发感染（R2 = 0.970）、疾病严重程度（R2 = 0.776）、叶片裂苔发育（R2 = 0.455）呈显著相关。结果表明，该方法可用于对chasmothecia进行定量，改进现有的基于目测计数的方法，证明PM流行与chasmothecia之间的相互关系，以及改进PM疾病预测模型和随后的杀菌剂应用。快速和容易的子囊菌接种量的量化将极大地促进控制行动对不同水平子囊菌接种量所造成的风险的协调。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytopathology 生物-植物科学

CiteScore

5.90

自引率

9.40%

发文量

505

审稿时长

4-8 weeks

期刊介绍： Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.