{"title":"Proteomic Analysis Reveals Differentially Expressed Proteins in Cordyceps militaris Cultured with Different Media.","authors":"Qiurong Jia, Jiahui Zhang, Shenghou Wang, Fangxu Xu","doi":"10.1007/s00284-024-04005-z","DOIUrl":null,"url":null,"abstract":"<p><p>Cordyceps militaris is rich in high quality protein, which is an excellent protein supplement. In this study, proteins were extracted from C. militaris cultured with tussah pupa and C. militaris cultured with wheat and analyzed by liquid chromatography coupled with mass spectrometer. Results showed that a total of 83 differentially expressed proteins (DEPs) were identified. KEGG analysis showed that the number of DEPs involved in amino sugar and nucleotide sugar metabolism and metabolic pathways was the largest. The expression levels of chitinase, tubulin alpha chain and heat shock 70 kDa protein were upregulated in C. militaris cultured with tussah pupa, and these key DEPs were mainly related to immune modulation and disease resistance. The results revealed the nutritional and functional differences in the fruiting bodies of C. militaris cultured with tussah pupa and wheat, respectively. The findings provide a theoretical basis for further studies on the biological function of proteins in C. militaris.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"29"},"PeriodicalIF":2.3000,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00284-024-04005-z","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Cordyceps militaris is rich in high quality protein, which is an excellent protein supplement. In this study, proteins were extracted from C. militaris cultured with tussah pupa and C. militaris cultured with wheat and analyzed by liquid chromatography coupled with mass spectrometer. Results showed that a total of 83 differentially expressed proteins (DEPs) were identified. KEGG analysis showed that the number of DEPs involved in amino sugar and nucleotide sugar metabolism and metabolic pathways was the largest. The expression levels of chitinase, tubulin alpha chain and heat shock 70 kDa protein were upregulated in C. militaris cultured with tussah pupa, and these key DEPs were mainly related to immune modulation and disease resistance. The results revealed the nutritional and functional differences in the fruiting bodies of C. militaris cultured with tussah pupa and wheat, respectively. The findings provide a theoretical basis for further studies on the biological function of proteins in C. militaris.
期刊介绍：
Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment.
Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas:
physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
