Regulatory Role of Long Noncoding RNAs LINC00449 in Hepatocellular Carcinoma Mechanism and Prognosis Via Sponge miR-329-3p/KIF5A Axis.

IF 1.4 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Turkish Journal of Gastroenterology Pub Date : 2024-11-04 DOI:10.5152/tjg.2024.24297

Yangyang Tang, Jinhuang Lin, Xiaobin Chi, Yichuan Zhang, Tonghai Xing

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引用次数: 0

Abstract

Background/aims: Hepatocellular carcinoma (HCC) is a globally prevalent malignant tumor with high recurrence and mortality rates. Long noncoding RNAs (lncRNAs) have been utilized to investigate the progression of various cancers, including HCC. The objective of this research is to explore the mechanism and prognostic impact of lncRNA LINC00449 on HCC.

Materials and methods: LINC00449 and miR-329-3p levels in HCC were measured using RT-qPCR. The impact of high- or lowLINC00449 expression on survival was tested. CCK-8, plate cloning, flow cytometry, and Transwell assays were selected to assess the biological functions of HCC cells. Kaplan-Meier and multivariate Cox analyses were conducted to evaluate the potential of LINC00449 as a prognostic marker for HCC. The interaction between LINC00449, miR-329-3p, and KIF5A was investigated through luciferase activity assays.

Results: LINC00449 expression in HCC tissues was increased. Silencing of LINC00449 improved the survival prospects of patients with HCC. Moreover, LINC00449 targeting the miR-329-3p/KIF5A axis influences the progression of HCC.

Conclusion: LINC00449 may become a biomarker for the prognosis of HCC, and the LINC00449/miR-329-3p/KIF5A regulatory network mediates the deterioration of HCC.

查看原文本刊更多论文

长链非编码rna LINC00449通过海绵miR-329-3p/KIF5A轴在肝细胞癌机制和预后中的调节作用

背景/目的：肝细胞癌（HCC）是一种全球流行的恶性肿瘤，具有高复发率和高死亡率。长链非编码rna （lncRNAs）已被用于研究包括HCC在内的各种癌症的进展。本研究旨在探讨lncRNA LINC00449在HCC中的作用机制及预后影响。材料与方法：采用RT-qPCR检测肝癌组织中LINC00449和miR-329-3p水平。检测linc00449高、低表达对存活的影响。选择CCK-8、平板克隆、流式细胞术和Transwell检测来评估HCC细胞的生物学功能。通过Kaplan-Meier和多变量Cox分析来评估LINC00449作为HCC预后标志物的潜力。通过荧光素酶活性测定研究LINC00449、miR-329-3p和KIF5A之间的相互作用。结果：肝癌组织中LINC00449表达升高。LINC00449的沉默改善了HCC患者的生存前景。此外，靶向miR-329-3p/KIF5A轴的LINC00449影响HCC的进展。结论：LINC00449可能成为HCC预后的生物标志物，LINC00449/miR-329-3p/KIF5A调控网络介导HCC的恶化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Turkish Journal of Gastroenterology 医学-胃肠肝病学

CiteScore

1.90

自引率

0.00%

发文量

127

审稿时长

6 months

期刊介绍： The Turkish Journal of Gastroenterology (Turk J Gastroenterol) is the double-blind peer-reviewed, open access, international publication organ of the Turkish Society of Gastroenterology. The journal is a bimonthly publication, published on January, March, May, July, September, November and its publication language is English. The Turkish Journal of Gastroenterology aims to publish international at the highest clinical and scientific level on original issues of gastroenterology and hepatology. The journal publishes original papers, review articles, case reports and letters to the editor on clinical and experimental gastroenterology and hepatology.