Report of a Spontaneous Potato Monoploid Resulting from a Biparental Diploid Potato Cross

Abstract

Reduction to a haploid state followed by chromosome doubling is an established method for the production of inbred lines for diploid-hybrid true seed production. In potato, the focus on haploid induction has been to use haploid inducers to develop primary dihaploids from tetraploid breeding clones via maternal haploid induction. Further reduction of diploid breeding lines into true monoploids via haploid induction is also possible but has not been explored extensively. PL-4, a diploid potato line developed as an improved haploid inducer, was crossed as female in a limited number of crosses with a diploid breeding line derived from Solanum tuberosum Group Phureja-stenotomum. Embryo rescue resulted in two offspring below the guard cell count threshold, and SNP analysis identified one offspring as a monoploid derived from PL-4.