Noah Williams, Greg Kornelis, Ek Han Tan, Paul Collins
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引用次数: 0
Abstract
Reduction to a haploid state followed by chromosome doubling is an established method for the production of inbred lines for diploid-hybrid true seed production. In potato, the focus on haploid induction has been to use haploid inducers to develop primary dihaploids from tetraploid breeding clones via maternal haploid induction. Further reduction of diploid breeding lines into true monoploids via haploid induction is also possible but has not been explored extensively. PL-4, a diploid potato line developed as an improved haploid inducer, was crossed as female in a limited number of crosses with a diploid breeding line derived from Solanum tuberosum Group Phureja-stenotomum. Embryo rescue resulted in two offspring below the guard cell count threshold, and SNP analysis identified one offspring as a monoploid derived from PL-4.
还原到单倍体状态后再进行染色体加倍是二倍体杂交种真种子生产自交系的一种成熟方法。在马铃薯中，单倍体诱导的重点是利用单倍体诱导剂通过母本单倍体诱导从四倍体育种无性系发育初生二倍体。通过单倍体诱导将二倍体育种系进一步减少为真正的单倍体也是可能的，但尚未广泛探索。作为单倍体诱导剂改良而成的二倍体马铃薯品系PL-4，与产自茄（Solanum tuberosum Group Phureja-stenotomum）的二倍体选育系进行了有限数量的雌性杂交。胚胎挽救导致两个后代低于保护细胞计数阈值，SNP分析确定一个后代是来自PL-4的单倍体。
期刊介绍：
The American Journal of Potato Research (AJPR), the journal of the Potato Association of America (PAA), publishes reports of basic and applied research on the potato, Solanum spp. It presents authoritative coverage of new scientific developments in potato science, including biotechnology, breeding and genetics, crop management, disease and pest research, economics and marketing, nutrition, physiology, and post-harvest handling and quality. Recognized internationally by contributors and readership, it promotes the exchange of information on all aspects of this fast-evolving global industry.
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