Selenium nano particles versus nano vitamin D3 in modulating anastrozole-induced osteoporosis on the mandibular alveolar bone of albino rats

IF 1.8 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Stomatology Oral and Maxillofacial Surgery Pub Date : 2024-12-01 DOI:10.1016/j.jormas.2024.102181

Souhayla Gamal Elmalah , Rania Osama M. Mohsen , Rabab Hassan

{"title":"Selenium nano particles versus nano vitamin D3 in modulating anastrozole-induced osteoporosis on the mandibular alveolar bone of albino rats","authors":"Souhayla Gamal Elmalah , Rania Osama M. Mohsen , Rabab Hassan","doi":"10.1016/j.jormas.2024.102181","DOIUrl":null,"url":null,"abstract":"<div><h3>Aim</h3><div>Compare the efficiency of selenium nanoparticles (SeNPs) and Nano vitamin D3 in modulating alveolar bone osteoporosis induced by anastrozole in albino rats.</div></div><div><h3>Materials and methods</h3><div>28 female albino rats were randomly divided into 4 groups (7/group); Control group (GP I): rats were received distilled water orally. Anastrozole group (GP II): rats were orally given anastrozole (1 mg/kg/day) dissolved in distilled water. SeNP treated Anastrozole group (GP III): rats were orally given anastrozole with SeNP (0.5 mg/kg/day). Nano vitamin D3 treated Anastrozole group (GP IV): rats were orally given anastrozole with Nano vitamin D3 (40 IU/kg/day). After 4 weeks of drugs administration, mandibles were prepared and stained with hematoxylin & eosin, Masson trichrome and anti-osteocalcin antibody. New collagen area% in Masson trichrome and positive anti-osteocalcin osteoblasts count were measured and statistically analyzed.</div></div><div><h3>Results</h3><div>Deleterious changes in the alveolar bone were detected in Gp II, while being markedly improved in treated groups. Moreover, GP II demonstrated large areas of old collagen, with positive anti-osteocalcin immunoreactivity in some osteoblasts and few osteocytes, meanwhile, control and treated groups showed more predominant newly formed collagen with positive immunoreactivity in nearly all osteoblasts, osteocytes, and some areas of bone matrix. Statistically, Gp III and Gp IV showed the significantly highest newly formed collagen mean area% and positive osteoblasts count, then Gp I, while Gp II represented the least recorded data.</div></div><div><h3>Conclusions</h3><div>SeNPs and Nano Vitamin D3 improve the structural and cellular quality of mandibular alveolar bone in anastrozole-induced osteoporosis of albino rats.</div></div>","PeriodicalId":55993,"journal":{"name":"Journal of Stomatology Oral and Maxillofacial Surgery","volume":"126 3","pages":"Article 102181"},"PeriodicalIF":1.8000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Stomatology Oral and Maxillofacial Surgery","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2468785524004701","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Aim

Compare the efficiency of selenium nanoparticles (SeNPs) and Nano vitamin D3 in modulating alveolar bone osteoporosis induced by anastrozole in albino rats.

Materials and methods

28 female albino rats were randomly divided into 4 groups (7/group); Control group (GP I): rats were received distilled water orally. Anastrozole group (GP II): rats were orally given anastrozole (1 mg/kg/day) dissolved in distilled water. SeNP treated Anastrozole group (GP III): rats were orally given anastrozole with SeNP (0.5 mg/kg/day). Nano vitamin D3 treated Anastrozole group (GP IV): rats were orally given anastrozole with Nano vitamin D3 (40 IU/kg/day). After 4 weeks of drugs administration, mandibles were prepared and stained with hematoxylin & eosin, Masson trichrome and anti-osteocalcin antibody. New collagen area% in Masson trichrome and positive anti-osteocalcin osteoblasts count were measured and statistically analyzed.

Results

Deleterious changes in the alveolar bone were detected in Gp II, while being markedly improved in treated groups. Moreover, GP II demonstrated large areas of old collagen, with positive anti-osteocalcin immunoreactivity in some osteoblasts and few osteocytes, meanwhile, control and treated groups showed more predominant newly formed collagen with positive immunoreactivity in nearly all osteoblasts, osteocytes, and some areas of bone matrix. Statistically, Gp III and Gp IV showed the significantly highest newly formed collagen mean area% and positive osteoblasts count, then Gp I, while Gp II represented the least recorded data.

Conclusions

SeNPs and Nano Vitamin D3 improve the structural and cellular quality of mandibular alveolar bone in anastrozole-induced osteoporosis of albino rats.

查看原文本刊更多论文

纳米硒颗粒与纳米维生素D3对阿那曲唑诱导的白化大鼠下颌牙槽骨骨质疏松的调节作用。

目的：比较纳米硒（SeNPs）和纳米维生素D3对阿那曲唑致白化大鼠牙槽骨骨质疏松的调节作用。材料与方法：雌性白化大鼠28只，随机分为4组（7只/组）；对照组（GP I）：大鼠口服蒸馏水。阿那曲唑组（GP II）：大鼠口服阿那曲唑（1 mg/kg/d）溶于蒸馏水。SeNP治疗阿那曲唑组（GP III）：大鼠口服阿那曲唑加SeNP （0.5 mg/kg/d）。纳米维生素D3治疗阿那曲唑组（GP IV组）：大鼠口服阿那曲唑加纳米维生素D3 （40 IU/kg/d）。给药4周后，制备下颌骨并用苏木精伊红、马松三色和抗骨钙素抗体染色。测定马松三色新胶原面积%和抗骨钙素阳性成骨细胞计数，并进行统计学分析。结果：Gpⅱ组牙槽骨病变明显，治疗组牙槽骨病变明显改善。此外，GP II表现出大面积的旧胶原，在部分成骨细胞和少量骨细胞中表现出抗骨钙素免疫反应阳性，同时，对照组和治疗组几乎在所有成骨细胞、骨细胞和骨基质的部分区域中表现出更多的新形成胶原，免疫反应阳性。统计上，Gp III和Gp IV的新形成胶原平均面积%和成骨细胞阳性计数最高，其次是Gp I，而Gp II的记录数据最少。结论：SeNPs和纳米维生素D3可改善阿那曲唑所致白化大鼠下颌牙槽骨的结构和细胞质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊