circ-TTC17 Promotes Esophagus Squamous Cell Carcinoma Cell Growth, Metastasis, and Inhibits Autophagy-Mediated Radiosensitivity Through miR-145-5p/SIRT1 Axis.

IF 2.3 3区医学 Q3 ONCOLOGY

Thoracic Cancer Pub Date : 2025-01-01 Epub Date: 2024-12-02 DOI:10.1111/1759-7714.15494

Ying Liu, Shengmin Lan, Zhihui Duan

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引用次数: 0

Abstract

Background: Circular RNA (circRNA) plays a significant role in esophagus squamous cell carcinoma (ESCC) progression. Nevertheless, circ-TTC17 roles in ESCC have not fully understood.

Methods: The levels of circ-TTC17, miR-145-5p and sirtuin 1 (SIRT1) were determined using qRT-PCR. ESCC cell functions were examined by CCK8 assay, flow cytometry, transwell assay and colony formation assay. The relative protein levels of autophagy marker and SIRT1 were determined by western blot (WB). The interactions among circ-TTC17, miR-145-5p, and SIRT1 were verified by dual-luciferase reporter assay and RIP assay.

Results: circ-TTC17 was overexpressed and miR-145-5p was underexpressed in ESCC. circ-TTC17 knockdown restrained ESCC cell proliferation and metastasis, while enhance apoptosis and autophagy-mediated radiosensitivity. Circ-TTC17 could sponge miR-145-5p, and its inhibitor reversed the inhibitory effect of circ-TTC17 knockdown on ESCC cell progression. Additionally, SIRT1 was targeted by miR-145-5p, and SIRT1 overexpression abolished miR-145-5p-mediated the suppressive effect on ESCC cell progression. Also, circ-TTC17 interference reduced ESCC tumor growth via miR-145-5p/SIRT1 axis.

Conclusion: circ-TTC17 promoted ESCC cell growth, metastasis and inhibited autophagy-mediated radiosensitivity by miR-145-5p/SIRT1 axis.

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circ-TTC17通过miR-145-5p/SIRT1轴促进食管鳞状细胞癌细胞生长、转移并抑制自噬介导的放射敏感性

背景：环状RNA （circRNA）在食管鳞状细胞癌（ESCC）的进展中起着重要作用。然而，circ-TTC17在ESCC中的作用尚未完全了解。方法：采用qRT-PCR检测circ-TTC17、miR-145-5p、sirtuin 1 （SIRT1）水平。采用CCK8法、流式细胞术、transwell法和集落形成法检测ESCC细胞功能。western blot （WB）检测自噬标志物和SIRT1的相对蛋白水平。circ-TTC17、miR-145-5p和SIRT1之间的相互作用通过双荧光素酶报告基因实验和RIP实验验证。结果：在ESCC中circ-TTC17过表达，miR-145-5p过表达。circ-TTC17敲低抑制ESCC细胞增殖和转移，同时增强凋亡和自噬介导的放射敏感性。Circ-TTC17可以“海绵”miR-145-5p，其抑制剂逆转了Circ-TTC17敲低对ESCC细胞进展的抑制作用。此外，SIRT1被miR-145-5p靶向，SIRT1过表达消除了miR-145-5p介导的对ESCC细胞进展的抑制作用。此外，circ-TTC17干扰通过miR-145-5p/SIRT1轴降低ESCC肿瘤生长。结论：circ-TTC17通过miR-145-5p/SIRT1轴促进ESCC细胞生长、转移，抑制自噬介导的放射敏感性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Thoracic Cancer ONCOLOGY-RESPIRATORY SYSTEM

CiteScore

5.20

自引率

3.40%

发文量

439

审稿时长

2 months

期刊介绍： Thoracic Cancer aims to facilitate international collaboration and exchange of comprehensive and cutting-edge information on basic, translational, and applied clinical research in lung cancer, esophageal cancer, mediastinal cancer, breast cancer and other thoracic malignancies. Prevention, treatment and research relevant to Asia-Pacific is a focus area, but submissions from all regions are welcomed. The editors encourage contributions relevant to prevention, general thoracic surgery, medical oncology, radiology, radiation medicine, pathology, basic cancer research, as well as epidemiological and translational studies in thoracic cancer. Thoracic Cancer is the official publication of the Chinese Society of Lung Cancer, International Chinese Society of Thoracic Surgery and is endorsed by the Korean Association for the Study of Lung Cancer and the Hong Kong Cancer Therapy Society. The Journal publishes a range of article types including: Editorials, Invited Reviews, Mini Reviews, Original Articles, Clinical Guidelines, Technological Notes, Imaging in thoracic cancer, Meeting Reports, Case Reports, Letters to the Editor, Commentaries, and Brief Reports.