RpoB H481Y Rifampicin Resistance Mutation-Associated Oxidative Stress Sensitivity Reduces the Virulence of Staphylococcus aureus

IF 1.9 4区医学 Q4 IMMUNOLOGY

Microbiology and Immunology Pub Date : 2024-12-01 DOI:10.1111/1348-0421.13190

Tomonori Kano, Kazuya Ishikawa, Lina Imai, Kazuyuki Furuta, Chikara Kaito

引用次数: 0

Abstract

In this study, we have established a Staphylococcus aureus RpoB H481Y mutant strain and demonstrated that it is sensitive to menadione or hydrogen peroxide-induced oxidative stress and exhibits reduced virulence against a silkworm infection model. Furthermore, the reduced virulence of the RpoB H481Y mutant was abrogated in the presence of N-acetylcysteine, a reactive oxygen species scavenger. These results suggest that oxidative stress sensitivity caused by the RpoB H481Y rifampicin resistance mutation attenuates the virulence of S. aureus.

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RpoB H481Y利福平耐药突变相关氧化应激敏感性降低金黄色葡萄球菌毒力

在这项研究中，我们建立了一株金黄色葡萄球菌RpoB H481Y突变株，并证明它对甲萘醌或过氧化氢诱导的氧化应激敏感，并对家蚕感染模型表现出降低的毒力。此外，在n -乙酰半胱氨酸（一种活性氧清除剂）的存在下，RpoB H481Y突变体的毒性降低被取消。这些结果表明，RpoB H481Y利福平耐药突变引起的氧化应激敏感性降低了金黄色葡萄球菌的毒力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiology and Immunology 医学-免疫学

CiteScore

5.20

自引率

3.80%

发文量

审稿时长

1 months

期刊介绍： Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.