A novel tape-free sample preparation method for human osteochondral cryosections for high throughput hyperspectral imaging.

IF 2.1 4区生物学 Q4 CELL BIOLOGY

Histochemistry and Cell Biology Pub Date : 2024-12-02 DOI:10.1007/s00418-024-02338-1

Xiwei Fan, Bogdan Donose, Michael W M Jones, Daryl Howard, Jari Torniainen, Karl Bertling, Xiao Guo, Cameron M Kewish, Kah Meng Lee, Antonia Rujia Sun, Aleksandar Rakic, Ross Crawford, Isaac O Afara, Indira Prasadam

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引用次数: 0

Abstract

Understanding the osteochondral junction, where non-mineralised cartilage and mineralised bone converge, is crucial for joint health. Current sample preparation techniques are insufficient for detailed spatial hyperspectral imaging analysis. Using the enhanced Kawamoto method, we used the super cryo embedding medium's temperature-dependent properties to transfer high-quality tissue samples onto slides for spatial imaging analysis. We transferred osteochondral samples using a tape-free system and successfully tested them in hematoxylin and eosin (HE), Safranin-O, nanomechanical assessments and nano-Fourier transform infrared (FTIR) mapping. This protocol elucidates the structural and elemental gradients, mechanical characteristics and distinctive biochemical layering, making it a useful tool for analysing biochemical properties' co-distribution in healthy and diseased situations.

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来源期刊

Histochemistry and Cell Biology 生物-细胞生物学

CiteScore

4.90

自引率

8.70%

发文量

112

审稿时长

1 months

期刊介绍： Histochemistry and Cell Biology is devoted to the field of molecular histology and cell biology, publishing original articles dealing with the localization and identification of molecular components, metabolic activities and cell biological aspects of cells and tissues. Coverage extends to the development, application, and/or evaluation of methods and probes that can be used in the entire area of histochemistry and cell biology.