{"title":"Sample Preparation Techniques for Analysis of Endocannabinoids in Biological Fluids and Tissues.","authors":"Demet Dincel, Yasin Darı","doi":"10.1080/10408347.2024.2432998","DOIUrl":null,"url":null,"abstract":"<p><p>This review discusses various sample pretreatment methods for the analytical assessment of endocannabinoids in biological fluids and tissues. Techniques like liquid-liquid extraction, protein precipitation, SALLE, SPME, and micro-solid phase extraction (µ-SPE) are investigated. The findings show that SALLE and SPME provide phase separation as a fast and environmentally friendly method and allow high sensitivity in the analyses. The analysis of endocannabinoids, particularly, 2-AG, is challenging due to its tendency to isomerize into 1-AG and its lack of stability. These challenges are highlighted, emphasizing the need for optimized analytical methods to ensure accurate and reliable results. In conclusion, this study demonstrates the applicability of effective extraction techniques as an alternative to traditional methods to obtain reliable results in endocannabinoid analyses and provides a new perspective to the literature.</p>","PeriodicalId":10744,"journal":{"name":"Critical reviews in analytical chemistry","volume":" ","pages":"1-12"},"PeriodicalIF":4.2000,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Critical reviews in analytical chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1080/10408347.2024.2432998","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
This review discusses various sample pretreatment methods for the analytical assessment of endocannabinoids in biological fluids and tissues. Techniques like liquid-liquid extraction, protein precipitation, SALLE, SPME, and micro-solid phase extraction (µ-SPE) are investigated. The findings show that SALLE and SPME provide phase separation as a fast and environmentally friendly method and allow high sensitivity in the analyses. The analysis of endocannabinoids, particularly, 2-AG, is challenging due to its tendency to isomerize into 1-AG and its lack of stability. These challenges are highlighted, emphasizing the need for optimized analytical methods to ensure accurate and reliable results. In conclusion, this study demonstrates the applicability of effective extraction techniques as an alternative to traditional methods to obtain reliable results in endocannabinoid analyses and provides a new perspective to the literature.
期刊介绍：
Critical Reviews in Analytical Chemistry continues to be a dependable resource for both the expert and the student by providing in-depth, scholarly, insightful reviews of important topics within the discipline of analytical chemistry and related measurement sciences. The journal exclusively publishes review articles that illuminate the underlying science, that evaluate the field''s status by putting recent developments into proper perspective and context, and that speculate on possible future developments. A limited number of articles are of a "tutorial" format written by experts for scientists seeking introduction or clarification in a new area.
This journal serves as a forum for linking various underlying components in broad and interdisciplinary means, while maintaining balance between applied and fundamental research. Topics we are interested in receiving reviews on are the following:
· chemical analysis;
· instrumentation;
· chemometrics;
· analytical biochemistry;
· medicinal analysis;
· forensics;
· environmental sciences;
· applied physics;
· and material science.
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