Analysis of vericiguat via ion-pairing with eosin Y as a spectrofluorimetric and spectrophotometric probe: Application to content uniformity test

Abstract

Using spectroscopy, quick and sensitive analytical methods based on Eosin Y ion pairing were developed and assessed in order to determine vericiguat with high selectivity and sensitivity. The drug is used for treating symptomatic chronic heart failure (HF), The quenching impact of vericiguat on the Eosin Y’s fluorescence at a pH 3.3, in 0.1 M acetate buffer solution was observed using spectrofluorometric technique. This technique is regarded as the original spectrofluorometric technique for the assay of vericiguat. The quenching effect on fluorescence was ranged from 100 to 1000 ng mL⁻¹. The absorbance of the generated ion-pair was measured using spectrophotometric method at 550 nm in aqueous buffered solutions with pH 3.3. In the concentration range of 1.0–10.0 µg mL⁻¹, Beer’s law was followed. The two approaches were applied to the analysis of dosage forms with a high percent recovery successfully, and they were assessed in compliance with ICH guidelines. Moreover, the method’s sustainability was evaluated and compared to the published method using two greenness assessment tools termed analytical eco-scale and Analytical GREENness (AGREE). That findings suggest that the method is more sustainable than the published method.