A land plant-specific VPS13 mediates polarized vesicle trafficking in germinating pollen

IF 8.1 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-12-01 DOI:10.1111/nph.20277

Surachat Tangpranomkorn, Yuka Kimura, Motoko Igarashi, Fumiko Ishizuna, Yoshinobu Kato, Takamasa Suzuki, Takuya Nagae, Sota Fujii, Seiji Takayama

引用次数: 0

Abstract

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陆地植物特异性VPS13介导萌发花粉的极化囊泡运输

花粉具有从干燥状态转变为极快生长状态的非凡能力。在授粉过程中，花粉从接触雌蕊处获得水分和Ca2+，这是花粉管萌发的方向线索。随后定向囊泡运输的快速激活必须支持花粉管的生长，但导致这一过程的分子机制在很大程度上是未知的。我们建立了一种基于荧光素酶的授粉试验，以筛选授粉后早期缺陷的基因突变体。我们鉴定了植物特异性的VPS13，拟南芥VPS13a在花粉萌发中起重要作用，并研究了其分子功能。AtVPS13a突变严重影响花粉萌发和粗内质网脂滴排出。AtVPS13a和分泌囊泡标记物的细胞积累模式在极化位点同步，对局部Ca2+升高有轻微延迟。在花粉水化开始后，我们发现了一个短暂的Ca2+穗状突起，这可能与花粉管形成的方向线索有关。尽管授粉后的Ca2+动态不受AtVPS13a缺失的影响，但突变体在花粉萌发过程中细胞壁沉积减少。AtVPS13a介导花粉极化，通过Ca2+信号调节适当的定向囊泡运输，促进定向管的生长。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.