Selection of Plastic-Binding DNA Aptamers for Microplastics Detection

IF 16.1 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Dr. Mohamad Zandieh, Dr. Xin Luo, Dr. Yu Zhao, Prof. Chuanliang Feng, Prof. Juewen Liu
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Abstract

Plastics are critical materials for modern technological applications, yet environmental contamination by microplastics has become a growing concern. In this study, DNA aptamers were isolated for two of the most abundant plastic materials: polyvinylchloride (PVC) and polystyrene (PS). These aptamers contain approximately 90 % cytosine and thymine but only 10 % purine content. Among them, the PVC-1 aptamer binds to PVC with a six-fold higher capacity than a random sequenced DNA. Among the tested plastic materials, PVC and PS exhibited the highest specific binding capacity. Using fluorophore-labeled PVC-1 aptamer, PS/PVC microplastics as low as 1 mg were detected, and the aptamer was selective for microplastics over other environmentally relevant materials, such as silica. Molecular dynamics simulations indicated that the aptamer attempted to maximize contact with the plastic surface for adsorption. This plastic-binding aptamer is expected to find applications in environmental monitoring and has fundamental implications for surface-binding aptamers.

Abstract Image

用于微塑料检测的塑料结合DNA适体的选择
塑料是现代技术应用的关键材料,但微塑料对环境的污染日益受到关注。在这项研究中,分离了两种最丰富的塑料材料:聚氯乙烯(PVC)和聚苯乙烯(PS)的DNA适配体。这些适体含有约90%的胞嘧啶和胸腺嘧啶,但只有10%的嘌呤含量。其中,PVC-1适体与PVC的结合能力是随机测序DNA的6倍。在测试的塑料材料中,PVC和PS的比结合力最高。使用荧光团标记的PVC-1适配体,可以检测到低至1 mg的PS/PVC微塑料,并且该适配体对微塑料的选择性优于其他与环境相关的材料,如二氧化硅。分子动力学模拟表明,适体试图最大限度地与塑料表面接触以进行吸附。这种塑料结合适体有望在环境监测中找到应用,并对表面结合适体具有基本意义。
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来源期刊
CiteScore
26.60
自引率
6.60%
发文量
3549
审稿时长
1.5 months
期刊介绍: Angewandte Chemie, a journal of the German Chemical Society (GDCh), maintains a leading position among scholarly journals in general chemistry with an impressive Impact Factor of 16.6 (2022 Journal Citation Reports, Clarivate, 2023). Published weekly in a reader-friendly format, it features new articles almost every day. Established in 1887, Angewandte Chemie is a prominent chemistry journal, offering a dynamic blend of Review-type articles, Highlights, Communications, and Research Articles on a weekly basis, making it unique in the field.
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