Debunking the dogma that RecBCD nuclease destroys phage.

IF 3.3 3区生物学 Q2 GENETICS & HEREDITY

Genetics Pub Date : 2024-11-28 DOI:10.1093/genetics/iyae199

Clarence Zheng, Curtis Furukawa, Jerry Liu, Srishti Sankaran, Han Lin, Nidhi Munugeti, Meranda Wang, Gerald R Smith

引用次数: 0

Abstract

For decades, it has been repeatedly claimed that the potent bacterial helicase-nuclease RecBCD (exonuclease V) destroys foreign (non-self) DNA, such as that of phages, but repairs and recombines cellular (self) DNA. While this would constitute a strong host-survival mechanism, no phage destroyed by RecBCD is ever specified in those claims. To determine which phages are destroyed by RecBCD, we searched for phage isolates that grow on Escherichia coli ΔrecBCD but not on recBCD+. In contrast to the prevailing claim, we found none among >80 new isolates from nature and >80 from previous collections. Based on these and previous observations, we conclude that RecBCD repairs broken DNA that can recombine but destroys DNA that cannot recombine and recycles the nucleotides.

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揭穿 RecBCD 核酸酶会破坏噬菌体的谬论。

几十年来，人们一再声称，强效细菌螺旋酶核酸酶 RecBCD（外切核酸酶 V）会破坏外来（非自身）DNA，如噬菌体的 DNA，但会修复和重组细胞（自身）DNA。虽然这将构成一种强大的宿主生存机制，但在这些声明中却从未明确指出 RecBCD 能消灭哪些噬菌体。为了确定哪些噬菌体会被 RecBCD 破坏，我们搜索了在大肠杆菌 ΔrecBCD 上生长而不在 recBCD+ 上生长的噬菌体分离物。与普遍的说法相反，我们从自然界新分离的超过 80 个噬菌体和以前收集的超过 80 个噬菌体中没有发现任何噬菌体。根据这些观察结果和以前的观察结果，我们得出结论：RecBCD 可修复能重组的断裂 DNA，但会破坏不能重组的 DNA 并回收核苷酸。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetics GENETICS & HEREDITY-

CiteScore

6.90

自引率

6.10%

发文量

177

审稿时长

1.5 months

期刊介绍： GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.