Julie M. Noah, Mathilde Gorse, Carole-Anne Romain, Elise J. Gay, Thierry Rouxel, Marie-Hélène Balesdent, Jessica L. Soyer
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引用次数: 0
Abstract
Leptosphaeria maculans is one of the major fungal pathogens on oilseed rape (Brassica napus), causing stem canker disease. The closely related Brassica species B. nigra, B. juncea, and B. carinata display extreme resistance toward stem canker. In this study, we demonstrate the nonhost status of B. carinata toward L. maculans in France through field experiments and inoculations performed in controlled conditions. A few isolates moderately adapted to B. carinata in controlled conditions were recovered in the field on B. nigra leaves, allowing us to investigate the unusual B. carinata–L. maculans interactions using molecular, macroscopic, and microscopic analyses. A cross between a L. maculans isolate adapted to B. napus and an isolate moderately adapted to B. carinata allowed the generation, in the lab, of recombinant L. maculans strains better adapted to B. carinata than the natural parental isolate obtained from B. nigra, and highlighted the polygenic determinism of the adaptation of L. maculans to B. carinata and B. napus. This biological material will allow further investigation of the molecular determinants of the adaptation of L. maculans to nonhost species and elucidate the genetic resistance basis of B. carinata.
Leptosphaeria maculans 是油菜（Brassica napus）上的主要真菌病原体之一，可引起茎腐病。密切相关的芸苔属物种 B. nigra、B. juncea 和 B. carinata 对茎腐病具有极强的抗性。在这项研究中，我们通过田间试验和在受控条件下接种，证明了 B. carinata 在法国对 L. maculans 的非宿主地位。在受控条件下，一些对 B. carinata 有适度适应性的分离株在田间的黑叶木（B. nigra）叶片上被发现，这使我们能够利用分子、宏观和微观分析来研究 B. carinata 与 L. maculans 之间不寻常的相互作用。通过将适应油菜的 L. maculans 分离物与适度适应荠菜的分离物杂交，在实验室中产生了比从黑麦草中获得的天然亲本分离物更适应荠菜的重组 L. maculans 株系，并突出了 L. maculans 对荠菜和油菜适应的多基因决定性。这些生物材料将有助于进一步研究 L. maculans 对非宿主物种适应性的分子决定因素，并阐明 B. carinata 的遗传抗性基础。
期刊介绍：
The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side.
Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following:
the structure, activities and communal behaviour of microbial communities
microbial community genetics and evolutionary processes
microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors
microbes in the tree of life, microbial diversification and evolution
population biology and clonal structure
microbial metabolic and structural diversity
microbial physiology, growth and survival
microbes and surfaces, adhesion and biofouling
responses to environmental signals and stress factors
modelling and theory development
pollution microbiology
extremophiles and life in extreme and unusual little-explored habitats
element cycles and biogeochemical processes, primary and secondary production
microbes in a changing world, microbially-influenced global changes
evolution and diversity of archaeal and bacterial viruses
new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.
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