MMP-9 expression in rat pup incisor teeth is not altered by maternal hypertension or maternal atenolol treatment during pregnancy and lactation

IF 2.9 4区生物学 Q3 CELL BIOLOGY

Journal of Molecular Histology Pub Date : 2024-11-29 DOI:10.1007/s10735-024-10294-2

Gracieli Prado Elias, Haylla de Faria Horta, Alanna Ramalho Mateus, Laura Valentina Borges Pes, Adrielle Ouchi Lopes, Fernando Chiba, Alberto Carlos Botazzo Delbem, Kikue Takebayashi Sassaki, Roberta Okamoto, Cristina Antoniali

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引用次数: 0

Abstract

Hypertension alters tooth formation and Atenolol reduces the blood pressure of spontaneously hypertensive rats (SHR) during pregnancy and lactation, and as demonstrated before, increases the microhardness of the SHR offspring’s teeth. MMP-9 is overexpressed in different tissues of hypertensive animals and treatment of hypertension substances can reverse this alteration. We hypothesize hypertension alters the expression of MMP-9 in dental structures of SHR offspring and that treating female SHR with atenolol prevents this alteration. This study aimed to evaluate the expression of matrix metalloproteinase (MMP-9) in incisor teeth (IT) in male offspring of SHR (30 days old) treated or untreated with Atenolol during pregnancy and lactation. MMP-9 expression was evaluated in ameloblasts (AM), enamel matrix (EM), odontoblasts (OD), and predentin (PD) of IT through immunohistochemical reactions (immunoperoxidase). Data were analyzed by Shapiro-Wilk and Kruskal-Wallis (p < 0.05), with Dunn post-test. Histological differences were not observed between IT tissues of SHR and normotensive Wistar rats. For the first time, our data showed that MMP-9 expression in specific dental structures is not altered in SHR. Atenolol treatment increased MMP-9 immunostaining in EM of Wistar rat, however, Atenolol did not alter MMP-9 in the IT tissues of SHR. Our results demonstrated that MMP-9 expression in dental tissues is not affected by hypertension or atenolol treatment in dental tissues. If confirmed in humans, the results obtained in this study will corroborate the suggestion that MMP-9 is not a viable therapeutic target for the treatment of dental alterations associated with maternal hypertension.

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大鼠幼崽门牙中 MMP-9 的表达不会因孕期和哺乳期母体高血压或母体阿替洛尔治疗而改变

高血压会改变牙齿的形成，阿替洛尔可降低妊娠期和哺乳期自发性高血压大鼠（SHR）的血压，并如前所述可增加 SHR 后代牙齿的微硬度。MMP-9在高血压动物的不同组织中过度表达，而高血压药物治疗可以逆转这种改变。我们假设高血压会改变MMP-9在SHR后代牙齿结构中的表达，而用阿替洛尔治疗雌性SHR可防止这种改变。本研究旨在评估妊娠期和哺乳期用阿替洛尔治疗或未用阿替洛尔治疗的雄性 SHR 后代（30 天大）切牙（IT）中基质金属蛋白酶（MMP-9）的表达。通过免疫组化反应（免疫过氧化物酶）评估了MMP-9在门牙釉母细胞（AM）、釉基质（EM）、牙本质母细胞（OD）和前牙素（PD）中的表达。数据经 Shapiro-Wilk 和 Kruskal-Wallis 分析（p < 0.05）和 Dunn 后检验。在 SHR 和正常血压 Wistar 大鼠的 IT 组织之间未观察到组织学差异。我们的数据首次表明，MMP-9 在 SHR 特定牙齿结构中的表达没有改变。阿替洛尔治疗可增加 Wistar 大鼠 EM 中的 MMP-9 免疫染色，但阿替洛尔并未改变 SHR IT 组织中的 MMP-9。我们的研究结果表明，牙齿组织中 MMP-9 的表达不受高血压或阿替洛尔治疗牙齿组织的影响。如果在人体中得到证实，本研究获得的结果将证实MMP-9不是治疗与母体高血压相关的牙齿改变的可行治疗靶点这一观点。

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.