Establishment of a visual assessment platform for total antioxidant capacity of Ganoderma sichuanense based on Arg-CeO2 hydrogel nanozyme utilizing a self-programmed smartphone app and a self-designed 3D-printed device

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-11-29 DOI:10.1007/s00604-024-06840-0

Suhui Tan, Mengyuan Tan, Tao Zhang, Lu Gao, Yi Li, Xuechao Xu, Zhen-quan Yang

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引用次数: 0

Abstract

A multienzyme-like L-argininemodified CeO₂ (Arg-CeO₂) nanozyme was prepared for accurate total antioxidant capacity (TAC) determination of Ganoderma sichuanense (G. sichuanense). It exhibits oxidase-like and peroxidase-like activities catalyzing the generation of superoxide anion free radicals and hydroxyl radicals, accelerating 3, 3′, 5, 5′-tetramethylbenzidine (TMB) chromogenic reaction. Antioxidants can effectively eliminate the generated free radicals, inhibiting the TMB chromogenic reaction. Hence, TAC can be assessed by Arg-CeO₂ catalyzed TMB chromogenic reaction using ascorbic acid (AA) as a reference substance. TAC of G. sichuanense extracted using different solvents was determined, indicating the highest TAC observed in the G. sichuanense extracted by the mixture solvent (deionized water/ethanol/ethyl acetate = 1 : 1 : 1). A visual assessment platform based on Arg-CeO₂ hydrogel nanozyme utilizing a self-programmed smartphone app and a self-designed 3D printed device is established. TAC using AA as a reference substance (3.33 ~ 83.33 µM) can be detected with a detection limit as low as 0.58 µM. The platform can realize one-step quantitative determination of TAC in G. sichuanense within 40 min. The proposed visual assessment platform presented exhibits promising application prospects in the field of TAC assessment in food.

Graphical Abstract

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基于 Arg-CeO2 水凝胶纳米酶的灵芝总抗氧化能力可视化评估平台的建立--利用自编程智能手机应用程序和自行设计的 3D 打印设备

制备了一种多酶样 L-精氨酸修饰 CeO2（Arg-CeO2）纳米酶，用于准确测定四川灵芝的总抗氧化能力（TAC）。Arg-CeO2 纳米酶具有类似氧化酶和过氧化物酶的活性，能催化超氧阴离子自由基和羟自由基的生成，加速 3，3′，5，5′-四甲基联苯胺（TMB）显色反应。抗氧化剂能有效消除产生的自由基，抑制 TMB 致色反应。因此，可以用抗坏血酸（AA）作为参照物，通过 Arg-CeO2 催化的 TMB 致色反应来评估 TAC。测定了不同溶剂萃取的川贝母的 TAC，结果表明混合溶剂（去离子水/乙醇/乙酸乙酯 = 1 : 1 : 1）萃取的川贝母的 TAC 最高。基于 Arg-CeO2 水凝胶纳米酶，利用自编程智能手机应用程序和自行设计的 3D 打印设备，建立了一个可视化评估平台。以 AA 为参考物质（3.33 ~ 83.33 µM）检测 TAC，检测限低至 0.58 µM。该平台可在 40 分钟内实现川贝母中 TAC 的一步定量检测。所提出的可视化评估平台在食品中TAC评估领域具有广阔的应用前景。图文摘要

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.