Denitrifying Anaerobic Methane Oxidation Activity and Microbial Mechanisms in Riparian Zone Soils of the Yulin River, a Tributary of the Three Gorges Reservoir

IF 11.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2024-11-28 DOI:10.1016/j.watres.2024.122865

Shushan Hu, Qiang He, Yunan Liu, Cheng Cheng

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Abstract

Riparian zones are recognized as major sources of greenhouse gas emissions, particularly methane (CH₄). Denitrifying anaerobic methane oxidation (DAMO) has garnered growing attention due to its significant contribution to mitigating CH₄ emissions in wetland environments. Nonetheless, the specific role and microbial mechanisms of DAMO in controlling CH₄ release within riparian zones are still not well comprehended. This study employed isotopic labeling experiments to measure the nitrate-dependent anaerobic methane oxidation (NaDAMO) and nitrite-dependent anaerobic methane oxidation (NiDAMO) potential of soil samples from riparian zones that were collected during different hydrological cycles. Moreover, soil physicochemical properties, DAMO activity, and microbial abundance were integrated to analyze the key factors and mechanisms influencing DAMO in riparian zone soils. The isotope tracer results showed that NaDAMO activities (1.4–11.93 nmol ¹³CO₂ g^-1day^-1) were significantly higher than NiDAMO activities (0.66–9.19 nmol ¹³CO₂ g^-1day^-1) in the riparian zone (p<0.05). NiDAMO activities were more strongly influenced by hydrological variations compared to NaDAMO activities, exhibiting higher levels during the discharge period (2.78–9.19 nmol ¹³CO₂ g^-1day^-1) compared to the impoundment period (0.66–4.10 nmol ¹³CO₂ g^-1day^-1). The qPCR analysis showed that the gene copies of NaDAMO archaeal mcrA (10⁷ copies g^-1) were approximately ten times greater than those of NiDAMO bacterial pmoA (10⁶ copies g^-1) in the majority of the sampling sites. Correlation analyses revealed that NiDAMO activity was influenced by soil pH (p<0.05), while NaDAMO microbes were influenced by temperature, organic carbon, and ammonia nitrogen concentrations (p<0.05). In summary, this research explored how hydrological changes in the riparian zone influence DAMO activities and their underlying mechanisms, providing a theoretical basis for mitigating CH₄ emissions in riparian zones of reservoir regions.

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三峡库区支流榆林河河岸带土壤中的反硝化厌氧甲烷氧化活性和微生物机理

河岸带被认为是温室气体排放的主要来源，尤其是甲烷（CH4）。反硝化厌氧甲烷氧化（DAMO）在减少湿地环境中的甲烷排放方面做出了重大贡献，因此受到越来越多的关注。然而，人们对反硝化厌氧甲烷氧化（DAMO）在控制河岸带 CH4 释放方面的具体作用和微生物机制仍不甚了解。本研究采用同位素标记实验，测量了在不同水文周期采集的河岸带土壤样本的硝酸盐依赖性厌氧甲烷氧化（NaDAMO）和亚硝酸盐依赖性厌氧甲烷氧化（NiDAMO）潜力。此外，还综合了土壤理化性质、DAMO 活性和微生物丰度，分析了影响河岸带土壤中 DAMO 的关键因素和机制。同位素示踪结果表明，河岸带土壤中 NaDAMO 活性（1.4-11.93 nmol 13CO2 g-1day-1 ）显著高于 NiDAMO 活性（0.66-9.19 nmol 13CO2 g-1day-1 ）（p<0.05）。与 NaDAMO 活性相比，NiDAMO 活性受水文变化的影响更大，在排水期（2.78-9.19 nmol 13CO2 g-1day-1 ）比蓄水期（0.66-4.10 nmol 13CO2 g-1day-1 ）表现出更高的水平。qPCR 分析表明，在大多数采样点，NaDAMO 古菌 mcrA 的基因拷贝数（107 拷贝 g-1）比 NiDAMO 细菌 pmoA 的基因拷贝数（106 拷贝 g-1）高出约 10 倍。相关分析表明，NiDAMO 活性受土壤 pH 值的影响（p<0.05），而 NaDAMO 微生物受温度、有机碳和氨氮浓度的影响（p<0.05）。总之，本研究探讨了河岸带水文变化如何影响 DAMO 活性及其内在机制，为减轻库区河岸带的 CH4 排放提供了理论依据。

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.