Potentiometric enzymatic aptasensing of paralytic shellfish toxins using graphene-wrapped magnetic nanospheres and an all-solid-state cadmium-selective microelectrode

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2024-11-28 DOI:10.1016/j.snb.2024.137032

Jinghui Li, Wenting Zhang, Tanji Yin, Wei Qin

{"title":"Potentiometric enzymatic aptasensing of paralytic shellfish toxins using graphene-wrapped magnetic nanospheres and an all-solid-state cadmium-selective microelectrode","authors":"Jinghui Li, Wenting Zhang, Tanji Yin, Wei Qin","doi":"10.1016/j.snb.2024.137032","DOIUrl":null,"url":null,"abstract":"A highly sensitive potentiometric enzymatic aptasensing system for the detection of paralytic shellfish toxins (PSTs) is developed by integrating graphene-wrapped magnetic nanospheres (Fe3O4@SiO2@rGO) with an all-solid-state ion-selective microelectrode. The multilayered core-shell structure of Fe3O4@SiO2@rGO enhances the efficient adsorption of aptamer molecules tagged with cadmium sulfide (CdS) nanoparticles. These CdS-aptamer conjugates, when bound with PST molecules, can be desorbed from the Fe3O4@SiO2@rGO surface into the sample solution and subsequently digested by deoxyribonuclease I (DNase I). This digestion process releases the PST molecules for recirculation, significantly reducing the retention of CdS on the surface of Fe3O4@SiO2@rGO. After magnetic separation and acid dissolution, the released Cd2+ from the magnetic nanospheres are measured using an all-solid-state Cd2+-selective microelectrode, enabling sensitive potentiometric detection of PSTs. Using gonyautoxin 1/4 (GTX 1/4) as a model, the sensing system exhibits a linear concentration range from 5 to 150 pM and a low detection limit of 1.7 pM for GTX 1/4, and has been successfully applied in the analysis of seawater samples.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"71 1","pages":""},"PeriodicalIF":8.0000,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors and Actuators B: Chemical","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.snb.2024.137032","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

A highly sensitive potentiometric enzymatic aptasensing system for the detection of paralytic shellfish toxins (PSTs) is developed by integrating graphene-wrapped magnetic nanospheres (Fe₃O₄@SiO₂@rGO) with an all-solid-state ion-selective microelectrode. The multilayered core-shell structure of Fe₃O₄@SiO₂@rGO enhances the efficient adsorption of aptamer molecules tagged with cadmium sulfide (CdS) nanoparticles. These CdS-aptamer conjugates, when bound with PST molecules, can be desorbed from the Fe₃O₄@SiO₂@rGO surface into the sample solution and subsequently digested by deoxyribonuclease I (DNase I). This digestion process releases the PST molecules for recirculation, significantly reducing the retention of CdS on the surface of Fe₃O₄@SiO₂@rGO. After magnetic separation and acid dissolution, the released Cd²⁺ from the magnetic nanospheres are measured using an all-solid-state Cd²⁺-selective microelectrode, enabling sensitive potentiometric detection of PSTs. Using gonyautoxin 1/4 (GTX 1/4) as a model, the sensing system exhibits a linear concentration range from 5 to 150 pM and a low detection limit of 1.7 pM for GTX 1/4, and has been successfully applied in the analysis of seawater samples.

查看原文本刊更多论文

使用石墨烯包裹的磁性纳米球和全固态镉选择性微电极对麻痹性贝类毒素进行电位计酶促感应

通过将石墨烯包裹的磁性纳米球（Fe3O4@SiO2@rGO）与全固态离子选择性微电极整合在一起，开发出了一种用于检测麻痹性贝类毒素（PSTs）的高灵敏度电位酶联反应系统。Fe3O4@SiO2@rGO 的多层核壳结构提高了硫化镉（CdS）纳米颗粒标记的适配体分子的吸附效率。这些与 PST 分子结合的 CdS-aptamer 共轭物可以从 Fe3O4@SiO2@rGO 表面解吸到样品溶液中，然后被脱氧核糖核酸酶 I（DNase I）消化。这一消化过程释放出 PST 分子进行再循环，大大减少了 CdS 在 Fe3O4@SiO2@rGO 表面的保留。在磁分离和酸溶解之后，利用全固态 Cd2+ 选择性微电极测量磁性纳米球释放的 Cd2+，从而实现对 PST 的灵敏电位检测。该传感系统以龙葵毒素 1/4（GTX 1/4）为模型，其线性浓度范围为 5 至 150 pM，GTX 1/4的检测限低至 1.7 pM，并已成功应用于海水样品的分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.