Transcriptomic data of peach varieties with different chilling requirement levels.

IF 1.9 Q3 GENETICS & HEREDITY
Weihan Zhang, Yayun Sun, Haiyan Li, Yuepeng Han, Baoxiong Wan, Liao Liao
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引用次数: 0

Abstract

Objectives: Peach is a deciduous tree widely cultivated in temperate and subtropical regions that requires a process of bud endodormancy to produce normal flowering and fruiting. This release requires a certain accumulation of cold, named chilling requirement (CR). CR is genotype dependent and with varies levels among different species and accessions. Thus, we collected the bud transcriptomic data of two peaches with different CR levels and conduct a series standard basic analysis. The peach bud transcriptomic data we gathered provides a valuable dataset for exploring the relationships between gene expression and peach CR levels.

Data description: We extracted and sequenced the RNA of different CR peach buds at the same status in three endodormancy stages. Each stages have three biological replicates. A total of 18 RNA-seq libraries were obtained and mapped to the reference genome after quality control. The gene expression level was normalized by two methods (TPM and FPKM). Differentially expressed genes (DEGs) analysis revealed that a total of 2,481 unique genes with an absolute value of log2 fold change (FC) greater than 1.0. Homologous functional annotation of these DEGs were conducted which provided further information for CR potential related genes identified and functional genomics studies.

具有不同冷冻需求水平的桃品种的转录组数据。
目的:桃是一种广泛栽培于温带和亚热带地区的落叶乔木,需要经过花芽内眠过程才能正常开花结果。这一过程需要一定的低温积累,即冷冻要求(CR)。CR与基因型有关,不同物种和品种的CR水平不同。因此,我们收集了两种不同CR水平的桃的花蕾转录组数据,并进行了一系列标准的基本分析。我们收集的桃芽转录组数据为探索基因表达与桃CR水平之间的关系提供了宝贵的数据集:我们提取并测序了三个萌发末期处于相同状态的不同 CR 桃芽的 RNA。每个阶段有三个生物重复。共获得 18 个 RNA-seq 文库,并在质量控制后映射到参考基因组。基因表达水平采用两种方法(TPM 和 FPKM)进行归一化。差异表达基因(DEGs)分析表明,共有 2481 个独特基因的对数折合变化(FC)绝对值大于 1.0。对这些 DEGs 进行了同源功能注释,为 CR 潜在相关基因的鉴定和功能基因组学研究提供了更多信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.90
自引率
0.00%
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