Assessment of Biofilm Formation and Anti-Inflammatory Response of a Probiotic Blend in a Cultured Canine Cell Model.

IF 4.1 2区生物学 Q2 MICROBIOLOGY

Microorganisms Pub Date : 2024-11-11 DOI:10.3390/microorganisms12112284

Nicholas L F Gallina, Nicole Irizarry Tardi, Xilin Li, Alvin Cai, Mandy J Horn, Bruce M Applegate, Lavanya Reddivari, Arun K Bhunia

{"title":"Assessment of Biofilm Formation and Anti-Inflammatory Response of a Probiotic Blend in a Cultured Canine Cell Model.","authors":"Nicholas L F Gallina, Nicole Irizarry Tardi, Xilin Li, Alvin Cai, Mandy J Horn, Bruce M Applegate, Lavanya Reddivari, Arun K Bhunia","doi":"10.3390/microorganisms12112284","DOIUrl":null,"url":null,"abstract":"Gut dysbiosis and an inflamed bowel are growing concerns in mammals, including dogs. Probiotic supplements have been used to restore the natural microbial community and improve gastrointestinal health. Biofilm formation, antimicrobial activities, and immunological responses of probiotics are crucial to improving gut health. Thus, we tested a commercial probiotic blend (LabMAX-3), a canine kibble additive comprising Lactobacillus acidophilus, Lacticaseibacillus casei, and Enterococcus faecium for their ability to inactivate common enteric pathogens; their ability to form biofilms; epithelial cell adhesion; and their anti-inflammatory response in the Madin-Darby Canine Kidney (MDCK) cell line. Probiotic LabMAX-3 blend or individual isolates showed a strong inhibitory effect against Salmonella enterica, Listeria monocytogenes, enterotoxigenic Escherichia coli, and Campylobacter jejuni. LabMAX-3 formed biofilms comparable to Staphylococcus aureus. LabMAX-3 adhesion to the MDCK cell line (with or without lipopolysaccharide (LPS) pretreatment) showed comparable adhesion and biofilm formation (p < 0.05) to L. casei ATCC 334 used as a control. LabMAX-3 had no cytotoxic effects on the MDCK cell line during 1 h exposure. The interleukin-10 (IL-10) and tumor necrosis factor alpha (TNFα) ratio of LabMAX-3, compared to the L. casei control, showed a significant increase (p < 0.05), indicating a more pronounced anti-inflammatory response. The data show that LabMAX-3, a canine kibble supplement, can improve gastrointestinal health.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 11","pages":""},"PeriodicalIF":4.1000,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microorganisms","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/microorganisms12112284","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Gut dysbiosis and an inflamed bowel are growing concerns in mammals, including dogs. Probiotic supplements have been used to restore the natural microbial community and improve gastrointestinal health. Biofilm formation, antimicrobial activities, and immunological responses of probiotics are crucial to improving gut health. Thus, we tested a commercial probiotic blend (LabMAX-3), a canine kibble additive comprising Lactobacillus acidophilus, Lacticaseibacillus casei, and Enterococcus faecium for their ability to inactivate common enteric pathogens; their ability to form biofilms; epithelial cell adhesion; and their anti-inflammatory response in the Madin-Darby Canine Kidney (MDCK) cell line. Probiotic LabMAX-3 blend or individual isolates showed a strong inhibitory effect against Salmonella enterica, Listeria monocytogenes, enterotoxigenic Escherichia coli, and Campylobacter jejuni. LabMAX-3 formed biofilms comparable to Staphylococcus aureus. LabMAX-3 adhesion to the MDCK cell line (with or without lipopolysaccharide (LPS) pretreatment) showed comparable adhesion and biofilm formation (p < 0.05) to L. casei ATCC 334 used as a control. LabMAX-3 had no cytotoxic effects on the MDCK cell line during 1 h exposure. The interleukin-10 (IL-10) and tumor necrosis factor alpha (TNFα) ratio of LabMAX-3, compared to the L. casei control, showed a significant increase (p < 0.05), indicating a more pronounced anti-inflammatory response. The data show that LabMAX-3, a canine kibble supplement, can improve gastrointestinal health.

查看原文本刊更多论文

在犬培养细胞模型中评估混合益生菌的生物膜形成和抗炎反应

肠道菌群失调和肠道发炎是包括狗在内的哺乳动物日益关注的问题。益生菌补充剂已被用于恢复天然微生物群落和改善胃肠道健康。益生菌的生物膜形成、抗菌活性和免疫反应对改善肠道健康至关重要。因此，我们测试了一种商用益生菌混合物（LabMAX-3），一种由嗜酸乳杆菌、酪酸乳杆菌和粪肠球菌组成的犬粮添加剂，以检测它们灭活常见肠道病原体的能力、形成生物膜的能力、上皮细胞粘附力以及在马丁-达比犬肾（MDCK）细胞系中的抗炎反应。益生菌 LabMAX-3 混合物或单个分离物对肠炎沙门氏菌、单核细胞增生李斯特菌、肠毒性大肠杆菌和空肠弯曲菌有很强的抑制作用。LabMAX-3 形成的生物膜与金黄色葡萄球菌相当。LabMAX-3对MDCK细胞系的粘附（无论是否经过脂多糖（LPS）预处理）显示出与作为对照的L. casei ATCC 334相当的粘附和生物膜形成（p < 0.05）。LabMAX-3在1小时的暴露过程中对MDCK细胞系没有细胞毒性作用。与L. casei对照组相比，LabMAX-3的白细胞介素-10（IL-10）和肿瘤坏死因子α（TNFα）比值显著增加（p < 0.05），表明抗炎反应更明显。数据表明，LabMAX-3作为一种犬粮补充剂，可以改善肠胃健康。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Microorganisms Medicine-Microbiology (medical)

CiteScore

7.40

自引率

6.70%

发文量

2168

审稿时长

20.03 days

期刊介绍： Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.