A New Genus Neotricholomopsis Gen. Nov and Description of Neotricholomopsis globispora Sp. Nov. (Phyllotopsidaceae, Agaricales) from Northwestern China Based on Phylogeny, Morphology, and Divergence Time.

Abstract

While investigating macrofungi diversity in Gansu province, northwestern China, five fresh and fleshy specimens were collected, which are characterized by nearly white to buff hemispherical pileus with waved margins, a disc depressed with coral to brownish red fibrillose scales, adnate to sub-decurrent lamellae with four relatively regular rows of lamellulae, a stipe that is central, hollow, frequently straight to curved; basidiospores that are globose to subglobose, 5.0-6.0 × (3.5-) 4.0-5.0 (-5.5) μm, narrowly clavate cheilocystidia predominantly, pleurocystidia and caulocystidia not observed; and a cutis pileipellis, with hyphae slightly inflated in the KOH. The results of phylogeny analysis indicated that the species forms an independent lineage in Phyllotopsidaceae based on the ITS (ITS5/ITS4) and nLSU (LR0R/LR7) dataset. Molecular clock analyses suggested the common ancestor of Neotricholomopsis emerged later than upper Cretaceous with a mean crown age of 229.36 Mya (95% highest posterior density of 129.63-343.08 Mya). These five specimens were described as an unreported taxon based on the phylogeny analysis combined with morphological examination and ecological and geographical distribution. Detailed descriptions, illustrations, and phylogenetic trees to demonstrate the placement of this species and discussions with its related species are provided.