Saussurea involucrata SiLEA5 Enhances Tolerance to Drought Stress in Solanum lycopersicum.

IF 4.7 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Foods Pub Date : 2024-11-15 DOI:10.3390/foods13223641

Xiaoyan Liu, Aowei Li, Guanghong Luo, Jianbo Zhu

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引用次数: 0

Abstract

Drought adversely affects plant growth, which leads to reduced crop yields and exacerbates food insecurity. Late embryogenesis abundant (LEA) proteins are crucial for plants' responses to abiotic stresses. This research further investigates the role of SiLEA5 by utilizing transgenic tomatoes under drought stress. The expression of SiLEA5 was upregulated under drought and abscisic acid (ABA) treatment, resulting in decreased electrolyte leakage and malondialdehyde content, alongside increased levels of osmotic regulators and antioxidant enzyme activity. These biochemical alterations reduce oxidative damage and enhance drought resistance. qRT-PCR analysis revealed the upregulation of ABA signaling genes and key enzymes involved in proline biosynthesis (P5CS) and dehydrin (DHN) synthesis under drought stress. Additionally, overexpression of SiLEA5 increased the net photosynthetic rate (Pn) and fruit yield of tomatoes by regulating stomatal density and aperture. These findings suggest that SiLEA5 may be a potential target for improving drought tolerance in tomatoes and other crops.

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Saussurea involucrata SiLEA5能增强番茄茄属植物对干旱胁迫的耐受性。

干旱会对植物生长产生不利影响，导致作物减产，并加剧粮食不安全状况。胚胎发生后期丰富蛋白（LEA）对植物应对非生物胁迫至关重要。本研究利用干旱胁迫下的转基因番茄进一步研究了 SiLEA5 的作用。在干旱和脱落酸（ABA）处理下，SiLEA5的表达上调，导致电解质渗漏和丙二醛含量降低，同时渗透调节剂水平和抗氧化酶活性提高。qRT-PCR 分析显示，在干旱胁迫下，ABA 信号基因以及参与脯氨酸生物合成（P5CS）和脱水素（DHN）合成的关键酶上调。此外，通过调节气孔密度和孔径，过表达 SiLEA5 提高了番茄的净光合速率（Pn）和果实产量。这些发现表明，SiLEA5 可能是提高番茄和其他作物耐旱性的潜在靶标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Foods Immunology and Microbiology-Microbiology

CiteScore

7.40

自引率

15.40%

发文量

3516

审稿时长

15.83 days

期刊介绍： Foods (ISSN 2304-8158) is an international, peer-reviewed scientific open access journal which provides an advanced forum for studies related to all aspects of food research. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists, researchers, and other food professionals to publish their experimental and theoretical results in as much detail as possible or share their knowledge with as much readers unlimitedly as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. There are, in addition, unique features of this journal: manuscripts regarding research proposals and research ideas will be particularly welcomed electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material we also accept manuscripts communicating to a broader audience with regard to research projects financed with public funds