Cytogenomic Characterization of Murine Neuroblastoma Cell Line Neuro-2a and Its Two Derivatives Neuro-2a TR-Alpha and Neuro-2a TR-Beta.

IF 5.1 2区生物学 Q2 CELL BIOLOGY

Cells Pub Date : 2024-11-15 DOI:10.3390/cells13221889

Lioba Hergenhahn, Niklas Padutsch, Shaymaa Azawi, Ralf Weiskirchen, Thomas Liehr, Martina Rinčic

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引用次数: 0

Abstract

Background: The Neuro-2a cell line, derived from a murine neuroblastoma (NB), was established as early as 1969 and originates from a transplantable tumor that arose spontaneously in an A/Jax male mouse in 1940. Since then, it has been applied in over 10,000 studies and is used by the World Organization for Animal Health for the routine diagnosis of rabies. Surprisingly, however, Neuro-2a has never been genetically characterized in detail; this study fills that gap.

Methods: The Neuro-2a cell line and two of its derivatives, Neuro-2a TR-alpha and Neuro-2a TR-beta, were analyzed for their chromosomal constitution using molecular cytogenetic approaches. Array comparative genomic hybridization was performed to characterize copy number alterations.

Results: Neuro-2A has a hyper-tetraploid karyotype with 70 to 97 chromosomes per cell, and the karyotypes of its two examined derivatives were quite similar. Neither of them had a Y-chromosome. The complex karyotype of Neuro-2a includes mitotically stable dicentres, neocentrics, and complex rearrangements resembling chromothripsis events. Although no amplification of euchromatin or oncogenes was detected, there are five derivative chromosomes with the amplification of centromere-near heterochromatic material and 1-5 additional derivatives consisting only of such material.

Conclusions: Since satellite DNA amplification has recently been found in advanced human tumors, this finding may be the corresponding equivalent in mice. An in silico translation of the obtained results into the human genome indicated that Neuro-2A is suitable as a model for advanced human NB.

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小鼠神经母细胞瘤细胞株 Neuro-2a 及其两种衍生物 Neuro-2a TR-Alpha 和 Neuro-2a TR-Beta 的细胞基因组特征。

背景：神经-2a细胞系源自小鼠神经母细胞瘤（NB），早在1969年就已建立，它源自1940年一只A/Jax雄性小鼠自发产生的可移植肿瘤。从那时起，它已被应用于 10,000 多项研究，并被世界动物卫生组织用于狂犬病的常规诊断。但令人惊讶的是，Neuro-2a从未被详细地描述过基因特征；本研究填补了这一空白：方法：使用分子细胞遗传学方法分析了 Neuro-2a 细胞系及其两个衍生物 Neuro-2a TR-α 和 Neuro-2a TR-beta的染色体构成。结果表明，Neuro-2a TR-alpha和Neuro-2a TR-beta的染色体结构都发生了改变：结果：Neuro-2A具有超四倍体核型，每个细胞有70至97条染色体。它们都没有 Y 染色体。Neuro-2a 的复杂核型包括有丝分裂稳定的二中心、新中心和类似于染色体三分裂事件的复杂重排。虽然没有检测到外染色质或癌基因的扩增，但有五条染色体的衍生物扩增了中心粒附近的异染色质，另有 1-5 条衍生物仅由此类物质组成：结论：由于最近在晚期人类肿瘤中发现了卫星 DNA 扩增，这一发现在小鼠中可能与之相当。将所获结果转化为人类基因组的硅学研究表明，Neuro-2A适合作为晚期人类NB的模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cells Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

9.90

自引率

5.00%

发文量

3472

审稿时长

16 days

期刊介绍： Cells (ISSN 2073-4409) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to cell biology, molecular biology and biophysics. It publishes reviews, research articles, communications and technical notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided.