CART (Cocaine- and Amphetamine-Regulated Transcript): A New Identified Intrafollicular Mediator in Ovulation Induction Protocols.

IF 3.9 3区工程技术 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomedicines Pub Date : 2024-11-13 DOI:10.3390/biomedicines12112598

Charalampos Voros, Despoina Mavrogianni, Sofoklis Stavros, Myrto Papamentzelopoulou, Evangelia Dimitroulia, Dimitrios Doumplis, Dimitris Mathiopoulos, Dimitrios Loutradis

{"title":"CART (Cocaine- and Amphetamine-Regulated Transcript): A New Identified Intrafollicular Mediator in Ovulation Induction Protocols.","authors":"Charalampos Voros, Despoina Mavrogianni, Sofoklis Stavros, Myrto Papamentzelopoulou, Evangelia Dimitroulia, Dimitrios Doumplis, Dimitris Mathiopoulos, Dimitrios Loutradis","doi":"10.3390/biomedicines12112598","DOIUrl":null,"url":null,"abstract":"Background/objectives: This study investigates the relationship between cocaine- and amphetamine-regulated transcript (CART) expression, leptin, and hormone profiles-specifically progesterone, testosterone, androstenedione, estradiol, follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG)-across four distinct ovulation induction protocols (HMG, HMG/hCG, rFSH, and rFSH/hCG). It also investigates the relationship between follicle-stimulating hormone receptor (FSHR) Ser680Asn polymorphisms, CART expression, and in vitro fertilization (IVF) results, with the goal of better understanding how CART and FSHR polymorphisms affect ovarian response and oocyte quality.Methods: Data were obtained from 94 women who underwent controlled ovarian stimulation (COS) as part of their IVF therapy. Hormone levels, CART expression, and FSHR polymorphisms were measured across all four ovulation induction procedures. Statistical studies were undertaken to investigate the relationships between CART expression, hormone levels, and IVF results.Results: The study found no significant difference in body mass index (BMI) amongst the four stimulation procedures (p-values varied from 0.244 to 0.909). CART expression did not show a significant correlation with hormone levels throughout the whole cohort (progesterone, testosterone, androstenedione, FSH, hCG, and estradiol; p > 0.05). However, CART levels were adversely linked with the number of follicles > 12 mm (r = -0.251, p = 0.018), total oocyte count (r = -0.247, p = 0.019), and oocyte maturity (r = -0.212, p = 0.048). Furthermore, there was a strong negative connection between CART expression and thyroid hormone T3 (r = -0.319, p = 0.048). Among FSHR polymorphisms, the SER/SER genotype was related to greater CART levels (mean 4.198 ± 2.257) than the SER/ASN and ASN/ASN genotypes (p = 0.031).Conclusions: These data indicate that CART expression and FSHR polymorphisms may influence ovarian response and oocyte quality in IVF patients, possibly acting as biomarkers for evaluating ovarian outcomes in various ovulation induction procedures.","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"12 11","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11592277/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedicines","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.3390/biomedicines12112598","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background/objectives: This study investigates the relationship between cocaine- and amphetamine-regulated transcript (CART) expression, leptin, and hormone profiles-specifically progesterone, testosterone, androstenedione, estradiol, follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG)-across four distinct ovulation induction protocols (HMG, HMG/hCG, rFSH, and rFSH/hCG). It also investigates the relationship between follicle-stimulating hormone receptor (FSHR) Ser680Asn polymorphisms, CART expression, and in vitro fertilization (IVF) results, with the goal of better understanding how CART and FSHR polymorphisms affect ovarian response and oocyte quality.

Methods: Data were obtained from 94 women who underwent controlled ovarian stimulation (COS) as part of their IVF therapy. Hormone levels, CART expression, and FSHR polymorphisms were measured across all four ovulation induction procedures. Statistical studies were undertaken to investigate the relationships between CART expression, hormone levels, and IVF results.

Results: The study found no significant difference in body mass index (BMI) amongst the four stimulation procedures (p-values varied from 0.244 to 0.909). CART expression did not show a significant correlation with hormone levels throughout the whole cohort (progesterone, testosterone, androstenedione, FSH, hCG, and estradiol; p > 0.05). However, CART levels were adversely linked with the number of follicles > 12 mm (r = -0.251, p = 0.018), total oocyte count (r = -0.247, p = 0.019), and oocyte maturity (r = -0.212, p = 0.048). Furthermore, there was a strong negative connection between CART expression and thyroid hormone T3 (r = -0.319, p = 0.048). Among FSHR polymorphisms, the SER/SER genotype was related to greater CART levels (mean 4.198 ± 2.257) than the SER/ASN and ASN/ASN genotypes (p = 0.031).

Conclusions: These data indicate that CART expression and FSHR polymorphisms may influence ovarian response and oocyte quality in IVF patients, possibly acting as biomarkers for evaluating ovarian outcomes in various ovulation induction procedures.

查看原文本刊更多论文

CART（可卡因和苯丙胺调节转录物）：在促排卵方案中新发现的一种卵泡内介质。

背景/目的：本研究调查了四种不同的排卵诱导方案（HMG、HMG/hCG、rFSH 和 rFSH/hCG）中可卡因和苯丙胺调控转录本（CART）表达、瘦素和激素谱（特别是孕酮、睾酮、雄烯二酮、雌二醇、卵泡刺激素（FSH）和人绒毛膜促性腺激素（hCG））之间的关系。该研究还调查了卵泡刺激素受体（FSHR）Ser680Asn多态性、CART表达和体外受精（IVF）结果之间的关系，目的是更好地了解CART和FSHR多态性如何影响卵巢反应和卵母细胞质量：方法：从94名接受控制性卵巢刺激（COS）作为体外受精治疗一部分的女性中获取数据。对所有四种促排卵过程中的激素水平、CART表达和FSHR多态性进行了测量。研究人员对 CART 表达、激素水平和试管婴儿结果之间的关系进行了统计研究：研究发现，在四种促排卵过程中，身体质量指数（BMI）没有明显差异（P 值从 0.244 到 0.909 不等）。CART的表达与整个人群的激素水平（孕酮、睾酮、雄烯二酮、FSH、hCG和雌二醇；P > 0.05）没有明显的相关性。然而，CART水平与大于12毫米的卵泡数（r = -0.251，p = 0.018）、卵母细胞总数（r = -0.247，p = 0.019）和卵母细胞成熟度（r = -0.212，p = 0.048）呈负相关。此外，CART表达与甲状腺激素T3（r = -0.319，p = 0.048）之间存在很强的负相关。在 FSHR 多态性中，SER/SER 基因型比 SER/ASN 和 ASN/ASN 基因型与更高的 CART 水平（平均 4.198 ± 2.257）相关（p = 0.031）：这些数据表明，CART表达和FSHR多态性可能会影响试管婴儿患者的卵巢反应和卵母细胞质量，可能成为评估各种促排卵过程中卵巢结果的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biomedicines Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology

CiteScore

5.20

自引率

8.50%

发文量

2823

审稿时长

8 weeks

期刊介绍： Biomedicines (ISSN 2227-9059; CODEN: BIOMID) is an international, scientific, open access journal on biomedicines published quarterly online by MDPI.