Oocyte holding and in vitro maturation duration between 28 and 34 hours do not affect equine OPU-ICSI outcomes

IF 2.4 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2024-11-22 DOI:10.1016/j.theriogenology.2024.11.019

Klaartje Broothaers , Osvaldo Bogado Pascottini , Mohamed Hedia , Daniel Angel-Velez , Tine De Coster , Sofie Peere , Ellen Polfliet , Emma Van den Branden , Jan Govaere , Ann Van Soom , Katrien Smits

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Abstract

Previous studies in the horse highlight the potential benefit of prolonged in vitro maturation (IVM) (34 h) compared to short IVM (24 h) with or without prior oocyte holding, but little is known about the optimal IVM duration within this interval. To determine the effect of oocyte holding and duration of IVM ranged between 28 and 34 h on nuclear maturation, cleavage, blastocyst formation, and pregnancy rates, a retrospective study was performed in an equine clinical OPU-ICSI setting. The study included data of 2114 aspirated oocytes from 201 OPU-ICSI sessions. Duration of IVM was divided in three different time windows using quartiles, with 465 oocytes (22.0 %) between 28 and 30 h (first quartile), 1078 oocytes (51.0 %) >30 and 31.7 h (second and third quartiles), and 571 oocytes (27.0 %) >31.7 and 34 h (fourth quartile). Using logistic regression models, the effect of duration of IVM with and without holding was tested on nuclear maturation, cleavage, blastocyst, and pregnancy rates. The three IVM intervals did not show differences in nuclear maturation (respectively 64.5 ± 0.48 %, 65.7 ± 0.47 %, and 67.3 ± 0.47 %), cleavage (respectively 59.7 ± 0.49 %, 58.5 ± 0.49 %, and 64.8 ± 0.48 %), blastocyst (respectively 17.5 ± 0.38 %, 19.0 ± 0.39 %, and 20.8 ± 0.41 %) nor pregnancy rates (respectively 65.4 ± 0.49 %, 70.3 ± 0.46 %, and 74.2 % ± 0.44) (P ≥ 0.38). Oocyte holding prior to IVM did not affect the results either (P ≥ 0.15). In conclusion, oocyte holding and IVM duration between 28 and 34h do not significantly affect outcomes, allowing flexibility in the planning of clinical OPU-ICSI in horses.

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28 至 34 小时的卵母细胞保留时间和体外成熟时间不会影响马 OPU-ICSI 的结果

之前在马身上进行的研究强调，与事先或不进行卵母细胞保温的短时间体外成熟（24 小时）相比，延长体外成熟（34 小时）可能会带来益处，但人们对这一时间间隔内的最佳体外成熟持续时间知之甚少。为了确定卵母细胞保留和 28 至 34 小时不等的 IVM 持续时间对核成熟、裂解、囊胚形成和妊娠率的影响，在马临床 OPU-ICSI 环境中进行了一项回顾性研究。该研究包括 201 次 OPU-ICSI 过程中抽取的 2114 个卵母细胞的数据。IVM 持续时间按四分法分为三个不同的时间窗口，其中 465 个卵母细胞（22.0%）在 28 至 30 小时之间（第一四分位），1078 个卵母细胞（51.0%）在 30 至 31.7 小时之间（第二和第三四分位），571 个卵母细胞（27.0%）在 31.7 至 34 小时之间（第四四分位）。利用逻辑回归模型，检验了有无保温的 IVM 持续时间对核成熟、分裂、囊胚和妊娠率的影响。三个 IVM 间隔在核成熟（分别为 64.5 ± 0.48 %、65.7 ± 0.47 % 和 67.3 ± 0.47 %）、卵裂（分别为 59.7 ± 0.49 %、58.5 ± 0.49 % 和 64.8 ± 0.48 %）、囊胚率（分别为 17.5 ± 0.38 %、19.0 ± 0.39 % 和 20.8 ± 0.41 %）和妊娠率（分别为 65.4 ± 0.49 %、70.3 ± 0.46 % 和 74.2 % ± 0.44 %）（P ≥ 0.38）。IVM 前的卵母细胞保留对结果也没有影响（P ≥ 0.15）。总之，28 至 34 小时之间的卵母细胞保留和 IVM 持续时间不会对结果产生显著影响，可灵活规划马匹的临床 OPU-ICSI 计划。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.