Genetic diversity and population structure associated with resistance to Phytophthora melonis-Pumpkin interaction pathosystem

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics Pub Date : 2024-11-23 DOI:10.1016/j.egg.2024.100309

Mohammadhadi Najafi , Mehdi Nasr Esfahani , Arman Nasr Esfahani , Kowsar Shirazii , Ava Nasr Esfahani , Hamid Akbarzadeh

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引用次数: 0

Abstract

Pumpkin (Cucurbita pepo), a versatile-member of the Cucurbitaceae-family, holds significant commercial-value in numerous countries, both as a cultivated-field-crop and a home-garden-staple. As with all crops, pumpkins are subject to diseases that can limit crop quality and yield. Considering the importance of the subject, in this research, SSR-markers were employed to investigate genetic-diversity and population-structure within a collection of 41 pumpkin-genotypes for the first time. At the same time, the screening for resistance to Phytophthora melonis, one of the important diseases of pumpkin globally, was carried out at greenhouse-conditions. In addition, defense-genes were analyzed against P. melonis, accordingly. The results showed that the pumpkin-genotypes have a high genetic-diversity. The 13-primers produced a total of 73-amplicons, of which 72 displayed polymorphic-patterns. Based on the dendrogram generated, the genotypes could be clustered into five distinct groups. Variance-analysis, showed that the related-genotypes had significant-reaction to the P. melonis. The genotypes were stratified into four groups based on their varying degrees of infection. (resistant, tolerant, sensitive and very sensitive) on the cut-off line found on the dendrogram. Comparison between the classification of genotypes in terms of disease-resistance and their grouping based on SSR-marker, there was a high agreement between the disease-severity and the dendrogram of the molecular-marker comprised their accordance with the disease-severity-percentage of the related pumpkin-genotyped accordingly. Moreover, the related defense-genes overexpressed following inoculation with to P. melonis, of which MYB, bHLH87 and ERF014 were more predominant significantly. The findings of this investigation elucidate the underlying mechanisms governing pumpkin resistance to Phytophthora root and crown rot. The data generated from this study can be leveraged to identify resistant pumpkin genotypes, promising SSR-marker and candidate genes for genetic enhancement of susceptible pumpkin cultivars and the subsequent development of P. melonis-resistant pumpkin varieties.

查看原文本刊更多论文

与抗甜瓜疫霉菌-南瓜交互病原系统有关的遗传多样性和种群结构

南瓜（Cucurbita pepo）是葫芦科植物中用途广泛的一种，在许多国家都具有重要的商业价值，既是田间栽培作物，也是家庭园艺主菜。与所有作物一样，南瓜也会受到病害的影响，从而限制作物的质量和产量。考虑到这一课题的重要性，本研究首次采用 SSR 标记研究 41 个南瓜基因型的遗传多样性和种群结构。同时，还在温室条件下筛选了南瓜对全球南瓜重要病害之一瓜疫霉（Phytophthora melonis）的抗性。此外，还相应地分析了南瓜的防御基因。结果表明，南瓜基因型具有很高的遗传多样性。13 个引物共产生 73 个扩增子，其中 72 个显示多态性模式。根据生成的树枝图，基因型可分为五个不同的组。方差分析显示，相关基因型对瓜皮病菌有显著的反应。根据不同的感染程度，基因型被分为四组。(根据树枝图上的分界线，基因型被分为四组（抗性组、耐受组、敏感组和极敏感组）。比较基因型的抗病性分类和基于 SSR 标记的分组，发现抗病性和分子标记的树枝状图与相关南瓜基因型的抗病性百分比高度一致。此外，接种甜瓜病菌后，相关的防御基因过度表达，其中 MYB、bHLH87 和 ERF014 的表达更为显著。本研究结果阐明了南瓜抗根腐病和冠腐病的内在机制。本研究产生的数据可用于鉴定抗性南瓜基因型、有希望的 SSR 标记和候选基因，以对易感南瓜栽培品种进行遗传改良，进而开发抗瓜皮病的南瓜品种。

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来源期刊

Ecological Genetics and Genomics Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： Ecological Genetics and Genomics publishes ecological studies of broad interest that provide significant insight into ecological interactions or/ and species diversification. New data in these areas are published as research papers, or methods and resource reports that provide novel information on technologies or tools that will be of interest to a broad readership. Complete data sets are shared where appropriate. The journal also provides Reviews, and Perspectives articles, which present commentary on the latest advances published both here and elsewhere, placing such progress in its broader biological context. Topics include: -metagenomics -population genetics/genomics -evolutionary ecology -conservation and molecular adaptation -speciation genetics -environmental and marine genomics -ecological simulation -genomic divergence of organisms