Transplantation of fecal microbiota from low to high residual feed intake chickens: Impacts on RFI, microbial community and metabolites profiles

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2024-11-23 DOI:10.1016/j.psj.2024.104567

Chunlin Xie, Qiying Liang, Jiaheng Cheng, Yushan Yuan, Lu Xie, Jian Ji

{"title":"Transplantation of fecal microbiota from low to high residual feed intake chickens: Impacts on RFI, microbial community and metabolites profiles","authors":"Chunlin Xie, Qiying Liang, Jiaheng Cheng, Yushan Yuan, Lu Xie, Jian Ji","doi":"10.1016/j.psj.2024.104567","DOIUrl":null,"url":null,"abstract":"<div><div>Improving feed efficiency is vital to bolster profitability and sustainability in poultry production. Although several studies have established links between gut microbiota and feed efficiency, the direct effects remain unclear. In this study, two distinct lines of Huiyang bearded chickens, exhibiting significant differences in residual feed intake (<strong>RFI</strong>), were developed after 15 generations of selective breeding. Fecal microbiota transplantation (<strong>FMT</strong>) from low RFI (<strong>LRFI</strong>) chickens to high RFI (<strong>HRFI</strong>) chickens resulted in a reduction trend in RFI, decreasing from 5.65 to 4.49 in the HRFI recipient chickens (<strong>HFMT</strong>). Microbiota composition and functional profiles in LRFI and HFMT chickens formed a distinct cluster compared to HRFI chickens. Using 16S rDNA sequencing and RandomForest analysis, <em>Slackia, Peptococcus, Blautia</em>, and <em>Dorea</em> were identified as key microbial markers associated with feed efficiency. Additionally, untargeted metabolomics identified common differential metabolites between HFMT and LRFI vs. HRFI groups. Correlation analysis showed significant correlations between these microbial markers and differential metabolites. These findings provide a foundation for microbiome-based strategies to improve feed efficiency in poultry.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 1","pages":"Article 104567"},"PeriodicalIF":3.8000,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0032579124011453","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Improving feed efficiency is vital to bolster profitability and sustainability in poultry production. Although several studies have established links between gut microbiota and feed efficiency, the direct effects remain unclear. In this study, two distinct lines of Huiyang bearded chickens, exhibiting significant differences in residual feed intake (RFI), were developed after 15 generations of selective breeding. Fecal microbiota transplantation (FMT) from low RFI (LRFI) chickens to high RFI (HRFI) chickens resulted in a reduction trend in RFI, decreasing from 5.65 to 4.49 in the HRFI recipient chickens (HFMT). Microbiota composition and functional profiles in LRFI and HFMT chickens formed a distinct cluster compared to HRFI chickens. Using 16S rDNA sequencing and RandomForest analysis, Slackia, Peptococcus, Blautia, and Dorea were identified as key microbial markers associated with feed efficiency. Additionally, untargeted metabolomics identified common differential metabolites between HFMT and LRFI vs. HRFI groups. Correlation analysis showed significant correlations between these microbial markers and differential metabolites. These findings provide a foundation for microbiome-based strategies to improve feed efficiency in poultry.

Abstract Image

查看原文本刊更多论文

将粪便微生物群从低残余饲料摄入量鸡移植到高残余饲料摄入量鸡：对 RFI、微生物群落和代谢物特征的影响

提高饲料效率对于提高家禽生产的盈利能力和可持续性至关重要。虽然已有多项研究确定了肠道微生物群与饲料效率之间的联系，但其直接影响仍不明确。在这项研究中，经过 15 代选择性育种，培育出了两个不同的惠阳胡须鸡品系，它们在剩余采食量（RFI）方面表现出显著差异。将粪便微生物群从低残余采食量（LRFI）鸡移植到高残余采食量（HRFI）鸡的粪便微生物群（FMT）导致残余采食量呈下降趋势，HRFI受体鸡的残余采食量从5.65降至4.49（HFMT）。与 HRFI 鸡相比，LRFI 和 HFMT 鸡的微生物群组成和功能特征形成了一个独特的群组。通过 16S rDNA 测序和 RandomForest 分析，发现 Slackia、Peptococcus、Blautia 和 Dorea 是与饲料效率相关的关键微生物标记。此外，非靶向代谢组学确定了 HFMT 和 LRFI 组与 HRFI 组之间常见的差异代谢物。相关分析表明，这些微生物标记物与差异代谢物之间存在明显的相关性。这些发现为基于微生物组的提高家禽饲料效率策略奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.