Microbiota composition of an autochthonous Krškopolje pig breed reared in two different organic production systems

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2024-11-25 DOI:10.1016/j.rvsc.2024.105449

Bojan Papić , Tim Šteferl , Jan Plut , Marina Štukelj

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引用次数: 0

Abstract

It has been shown that different production systems (conventional vs. pasture-raised pigs) and co-rearing of pigs with other livestock influence the gut microbiota composition in pigs. In this study, two independent trials were conducted to investigate the 16S fecal microbiota of the autochthonous Krškopolje pig. In each trial, three study groups were analyzed: (i) pasture-raised pigs cohabiting with small ruminants (group L1, farm 1), (ii) indoor-raised pigs without contact with other farm animals (group L2, farm 2) and fed the same commercial organic feed as the other two groups, and (iii) pasture-raised pigs cohabiting with cattle (group L3, farm 2). The pigs were sampled four times during the grower-finisher period, corresponding to the different seasons. A total of 18 and 22 pigs were included in trials 1 and 2, respectively. Alpha diversity was comparable between the study groups and the pigs of different age categories. The two predominant bacterial phyla in all three groups were Bacteroidota and Firmicutes. Significant differences in microbiota composition were found between pigs of different age categories in both trials (nonparametric MANOVA, p < 0.008). The microbiota composition of pigs from group L1 was significantly different from that of pigs from groups L2 and L3, whereas groups L2 and L3 tended to be more similar in both trials. The present results indicate that the trial and the farm of origin have a significant influence on the pig gut microbiota, and that their influence is more pronounced than that of the housing system.

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在两种不同有机生产系统中饲养的 Krškopolje 猪种的微生物群组成

研究表明，不同的生产系统（传统养猪与牧场养猪）以及猪与其他家畜共同饲养会影响猪的肠道微生物群组成。在本研究中，进行了两项独立试验，以调查自生的 Krškopolje 猪的 16S 粪便微生物群。在每个试验中，分析了三个研究小组：(i) 与小型反刍动物同居的牧场饲养猪（L1 组，1 号农场）；(ii) 不与其他农场动物接触的室内饲养猪（L2 组，2 号农场），喂食与其他两组相同的商业有机饲料；(iii) 与牛同居的牧场饲养猪（L3 组，2 号农场）。猪只在生长-成熟期采样四次，分别对应不同的季节。试验 1 和试验 2 分别包括 18 头和 22 头猪。研究组和不同年龄段的猪之间的α多样性相当。所有三个组中最主要的两个细菌门是类杆菌科和固醇菌科。在两次试验中，不同年龄组猪的微生物群组成存在显著差异（非参数 MANOVA，p < 0.008）。在两个试验中，L1 组猪的微生物群组成与 L2 组和 L3 组猪的微生物群组成存在显著差异，而 L2 组和 L3 组猪的微生物群组成则趋于相似。本研究结果表明，试验和原产猪场对猪肠道微生物群有很大影响，而且其影响比饲养系统的影响更为明显。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.