Roseateles microcysteis sp. nov., Isolated from Co-cultivation of Microcystis aeruginosa and Myriophyllum spicatum.

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2024-11-26 DOI:10.1007/s00284-024-04002-2

Min-Seong Kim, Seonah Jeong, Mingyeong Kang, So-Ra Ko, Ve Van Le, Dong-Yun Choi, Chi-Yong Ahn

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Abstract

A novel rod-shaped, Gram-stain-negative bacterial strain MS17^T was obtained from a co-culture of Microcystis aeruginosa and Myriophyllum spicatum. The examination of the 16S ribosomal RNA gene sequence showed a significant degree of similarity between strain MS17^T and Paucibacter sediminis S2-9^T (98.4%), Roseateles violae PFR6^T (98.1%), 'Roseateles cellulosilyticus' P8^T (98.0%), Roseateles aquae APW11^T (97.9%), Roseateles oligotrophus CHU3^T (97.7%), Roseateles saccharophilus DSM 654^T (97.6%), Kinneretia aquatilis CR182^T (97.7%), Pelomonas aquatica CCUG 52575^T (97.6%), and Roseateles toxinivorans 2C20^T (97.0%). Between strain MS17^T and the type strains of closely related species, digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values were below 21.9% and 78.48%. The respiratory quinone was ubiquinone Q-8. The main fatty acids (> 10.0%) were C_15:1 ω6c, C_16:0, C_17:0, and summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c). The polar lipids comprised phosphatidylethanolamine, unidentified phospholipid, phosphatidylglycerol, unidentified aminophospholipid, unidentified aminolipid, and three unidentified lipids. The genomic G + C content was 65.9%. According to phenotypic characteristics, phylogenetic relationships, and chemotaxonomic data, strain MS17^T has been categorized as a newly discovered species belonging to the Roseateles genus, and the name Roseateles microcysteis sp. nov. is suggested. It exhibits distinct biochemical properties that differentiate it from closely related species. The type strain is MS17^T (=KCTC 8001^T = LMG 33142^T).

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Roseateles microcysteis sp.

从铜绿微囊藻和藻类共培养物中获得了一株新型棒状革兰氏染色阴性菌株 MS17T。16S 核糖体 RNA 基因序列检查显示，菌株 MS17T 与 Paucibacter sediminis S2-9T （98.4%）、Roseateles violae PFR6T （98.1%）、'Roseateles cellulosilyticus' P8T （98.0%）、Roseateles aquae APW11T（97.9%）、Roseateles oligotrophus CHU3T（97.7%）、Roseateles saccharophilus DSM 654T（97.6%）、Kinneretia aquatilis CR182T（97.7%）、Pelomonas aquatica CCUG 52575T（97.6%）和 Roseateles toxinivorans 2C20T（97.0%）。MS17T菌株与近缘种的模式菌株之间的数字DNA-DNA杂交（dDDH）和平均核苷酸同一性（ANI）值分别低于21.9%和78.48%。呼吸醌为泛醌 Q-8。主要脂肪酸（> 10.0%）为 C15:1 ω6c、C16:0、C17:0 和总特征 3（C16:1 ω7c 和/或 C16:1 ω6c）。极性脂质包括磷脂酰乙醇胺、不明磷脂、磷脂酰甘油、不明氨基磷脂、不明氨基脂质和三种不明脂质。基因组 G + C 含量为 65.9%。根据表型特征、系统发育关系和化学分类学数据，菌株 MS17T 被归类为属于 Roseateles 属的新发现物种，建议命名为 Roseateles microcysteis sp.它表现出与近缘种不同的生化特性。模式菌株为 MS17T（=KCTC 8001T = LMG 33142T）。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.