Hybrid Structures Based on Silver Nanoparticles and Polyelectrolytes for Sensitive Fluorescent Solid-Phase Immunoassay

Abstract

The effect of surface modification of polystyrene plates for immunoassay with silver nanoparticles and polyelectrolytes on the fluorescent signal of adsorbed antibodies with fluorescent labels IgG-FITC, IgG-AF488 and IgG-AF647 was studied. The resulting solid phase was characterized by a sorption capacity increased by 30–50% as compared to high-binding polystyrene (Greiner, Austria) and the ability to enhance the fluorescent signals of the labels due to a plasmonic effect, which together increased the fluorescence intensity by 3–15 times, depending on the type of plate surface, the nature of the polyelectrolyte, and the optical and physicochemical properties of the labeled antibodies.